超氧化物歧化酶2基因rs4880位点单核苷酸多态性与男性不育发病风险的相关性研究

发布时间：2018-04-26 18:10

本文选题：超氧化物歧化酶 + rs　；参考：《中华男科学杂志》2017年02期

【摘要】：目的:探讨超氧化物歧化酶2(SOD2)基因rs4880位点单核苷酸多态性与男性不育发病风险的相关性。方法:采用病例-对照研究的方法,选取519例特发性男性不育患者作为病例组,年龄19~40(28.92±4.37)岁,并按精子浓度和前向运动(PR)精子百分率分为无精子症组(n=143)、严重少精子症组(n=175)、少精子症组(n=89)和弱精子症组(n=112)4个亚组;以338例正常生育的男性作为对照组,年龄19~40(28.40±4.25)岁,进行临床数据的采集。用Sequenom Mass Array技术对SOD2 rs4880位点进行基因分型,用Logistic回归模型分析SOD2 rs4880位点不同基因型与男性不育之间的关系。结果:病例组与正常对照组FSH、PR精子百分率、精子浓度存在显著差异(P0.01)。与野生型纯合TT比较,杂合突变型TC(OR=0.90,95%CI:0.65~1.25,P=0.516)与纯合突变型CC(OR=1.49,95%CI:0.38~5.81,P=0.566)均显示与男性不育无相关性;亚组分析中均显示该基因位点与男性不育不存在相关性:无精子症组中,TC/TT(OR=0.99.95%CI:0.62~1.58,P=0.967),CC/TT(OR=1.58,95%CI:0.26~9.59,P=0.619;严重少精子症组中,TC/TT(OR=1.07.95%CI:0.70~1.64,P=0.750),CC/TT(OR=1.31,95%CI:0.22~7.96,P=0.767;少精子症组中,TC/TT(OR=0.83.95%CI:0.47~1.48,P=0.535),CC/TT(OR=1.22,95%CI:0.13~11.90,P=0.865);弱精子症组中,TC/TT(OR=0.59.95%CI:0.33~1.05,P=0.074),CC/TT(OR=1.84,95%CI:0.30~11.16,P=0.510)。结论:SOD2 rs4880位点基因多态性与男性不育不存在相关性,但是由于实验样本条件的限制,需要更大的样本量以及样本选取范围来进一步研究验证。
[Abstract]:Objective: To investigate the correlation between the single nucleotide polymorphism of the superoxide dismutase 2 (SOD2) gene rs4880 locus and the risk of male infertility. Methods: a case control study was used to select 519 cases of idiopathic male infertility as case group, age 19~40 (28.92 + 4.37) years, and sperm percentage and forward movement (PR) sperm percentage. 4 subgroups were divided into azoospermia group (n=143), severe oligozoospermia group (n=175), oligozoospermia group (n=89) and asthenospermia group (n=112), and 338 normal fertile men were used as control group, and the age 19~40 (28.40 + 4.25) years were collected for clinical data. Sequenom Mass Array technique was used to genotyping SOD2 rs4880 loci, and Logistic return was used. The relationship between the different genotypes of SOD2 rs4880 loci and male infertility was analyzed. Results: there was a significant difference between the case group and the normal control group FSH, the percentage of PR sperm and the sperm concentration (P0.01). Compared with the wild type homozygous TT, the heterozygous mutant TC (OR=0.90,95%CI:0.65~1.25, P=0.516) and the homozygous mutant CC (OR=1.49,95%CI:0.38~5.81, P=0.5) 66) showed no correlation with male infertility; in subgroup analysis, there was no correlation between the gene locus and male infertility: TC/TT (OR=0.99.95%CI:0.62~1.58, P=0.967), CC/TT (OR=1.58,95%CI:0.26~9.59, P=0.619; severe oligozoospermia group, TC/TT (OR=1.07.95%CI:0.70~1.64, P=0.750), CC/TT (OR=1.31,95%CI:0.22~7) in azoospermia group. .96, P=0.767; TC/TT (OR=0.83.95%CI:0.47~1.48, P=0.535), CC/TT (OR=1.22,95%CI:0.13~11.90, P=0.865) in the oligozoospermia group; TC/TT (OR=0.59.95%CI:0.33~1.05, P=0.074) in the asthenospermia group. Conclusion: there is no correlation between the polymorphism of the gene and male infertility, but the experiment is due to the experiment The restriction of sample conditions requires more sample size and sample selection scope to further study and verify.

【作者单位】：江苏大学生命科学学院;南京军区南京总医院解放军检验医学研究所;江苏省人民医院临床生殖医学中心;江苏省计划生育科学技术研究所;
【基金】：江苏省科技厅省级科技专项(BM2015020) 南京市科技计划项目(201605004) 南京军区南京总医院院管题(2015046)~~
【分类号】：R698.2

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