茶树WRKY转录因子基因CsWRKY57的克隆及表达分析

发布时间：2018-04-27 02:23

本文选题：茶树 + CsWRKY　；参考：《茶叶科学》2017年04期

【摘要】：WRKY转录因子是植物特有的一类转录因子,在植物生长发育及胁迫应答过程中均发挥重要的调控作用。为探究WRKY转录因子与茶树抗旱及耐盐性的关系,本研究基于茶树转录组数据库中的检索结果,以陕茶1号1年生茶树为试验材料,克隆获得了1个WRKY转录因子基因,命名为CsWRKY57。生物信息学分析表明,CsWRKY57基因cDNA全长为1 222 bp,编码303个氨基酸,预测分子量为33.5 kD,理论等电点为5.49;另外,蛋白比对分析显示,CsWRKY57包含1个典型的WRKY核心序列和1个C2H2型锌指结构,属于WRKYIIc家族。实时荧光定量PCR分析结果显示,CsWRKY57基因在高盐、干旱、ABA胁迫下均被诱导表达,且表现出先增加后降低的趋势,表明CsWRKY57基因参与了茶树体内干旱、高盐和ABA的调控途径。转录激活活性试验表明,CsWRKY57无转录激活活性,意味着CsWRKY57可能需要与其他元件结合才能启动基因的表达。
[Abstract]:WRKY transcription factor is a kind of plant specific transcription factor, which plays an important regulatory role in plant growth and stress response. In order to explore the relationship between WRKY transcription factors and the resistance to drought and salt tolerance of tea tree, this study is based on the retrieval results in the tea tree transcriptional database. The 1 year old tea tree of Shanxi tea 1 was cloned as the experimental material. 1 WRKY transcription factor genes were obtained. The CsWRKY57. bioinformatics analysis showed that the full length of CsWRKY57 gene cDNA was 1222 BP, 303 amino acids were encoded, the predicted molecular weight was 33.5 kD, and the theoretical isoelectric point was 5.49. In addition, the protein comparison analysis showed that CsWRKY57 contains 1 typical WRKY core sequences and 1 C2H2 type zinc finger structures, belonging to WR. KYIIc family. Real-time fluorescence quantitative PCR analysis showed that the CsWRKY57 gene was induced under high salt, drought, and ABA stress, and showed a tendency to increase first and then decrease, indicating that the CsWRKY57 gene was involved in the regulation of drought, high salt and ABA in the tea plant. The transcriptional activation test showed that CsWRKY57 was no transcriptional activation activity, meaning that CsWRKY57 was no transcriptional activity. CsWRKY57 may need to integrate with other components to initiate gene expression.

【作者单位】：西北农林科技大学园艺学院;中国农业科学院茶叶研究所;
【基金】：陕西省茶叶产业技术体系(K3330215131) 陕西省科技统筹创新(KTZB02-01) 中央高校基本科研业务费专项资金(No.2452017074)
【分类号】：Q943.2;S571.1

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