盐胁迫下转复合多基因欧美杨107杨幼苗生长及生理响应

发布时间：2018-04-27 03:22

  本文选题：转复合多基因 + 欧美杨杨　； 参考：《林业科学》2017年07期

【摘要】：【目的】通过观测转复合多基因欧美杨107杨(转基因107杨)幼苗在不同浓度盐胁迫下生长性状,质膜透性、光合作用等各项指标,以及外源基因表达产物积累量的变化,综合评价转复合多基因杨树在盐胁迫下的适应能力。【方法】以转化成功且已经过验证的转基因107杨为试验材料,未转基因107杨为对照(CK),进行组培扩繁,在幼苗株高10 cm时定植在花盆中,置于人工气候室中培养。分别用0、3、6 g·L~(-1)的Na Cl溶液进行盐胁迫处理,胁迫25天后观测2个株系幼苗的生长指标、生理参数变化,以及甜菜碱与Bt毒蛋白的积累量。【结果】2个株系的生长指标观测结果表明:在低盐浓度处理下,转基因107杨幼苗株高增长量显著高于非转基因对照,转基因株系幼苗地径增长量与对照差异不显著;高盐浓度处理下,2个株系幼苗株高与地径均受到盐胁迫的显著影响。2个株系的生理参数观测结果表明:在低盐浓度处理下,转基因107杨幼苗质膜透性显著低于对照107杨,叶绿素含量有一定程度的增加,净光合速率与蒸腾速率显著高于对照,光系统Ⅱ实际光合效率Y(Ⅱ)小幅度降低但显著高于对照,光系统Ⅱ能够保持较高的电子传递速率;F_v/F_m增幅较高,具有较大光合作用潜力。在高盐浓度下,转基因株系幼苗叶绿素含量降幅较小,而质膜透性、净光合速率均显著降低,F_v/F_m、Y(Ⅱ)均呈现较大幅度下降,光系统Ⅱ电子传递速率受到较大影响,光合能力下降,变化趋势与对照相同,2个株系幼苗均受到较大程度的盐害。转基因株系中外源基因表达产物积累量的ELISA研究结果表明,随盐浓度的增加,Cry1Ac毒蛋白、Cry3A毒蛋白及甜菜碱含量都呈现增加趋势;在高盐浓度胁迫下外源基因产物积累量均显著增加。【结论】转基因107杨在低浓度盐胁迫下表现出良好的适应性,耐盐性优于对照107杨。在高浓度盐胁迫下转基因株系与对照107杨均受到较大影响,转基因株系并未显示其优势。盐胁迫可以诱导外源Bt基因、BADH基因表达加强,盐胁迫下转基因107杨表现出良好的耐盐和抗虫潜力。
[Abstract]:[objective] to observe the changes of growth traits, plasma membrane permeability, photosynthesis and accumulation of exogenous gene expression products in transgenic poplar 107 (transgenic poplar) seedlings under different concentrations of salt stress. The adaptability of transgenic poplar to salt stress was comprehensively evaluated. [methods] transgenic poplar 107, which had been successfully transformed and verified, was used as experimental material, and non-transgenic poplar 107 was used as control for tissue culture and propagation. The seedlings were planted in a flowerpot at a height of 10 cm and cultured in an artificial climate chamber. Salt stress was carried out in the NaCl solution of 0 ~ 3g / L ~ (-1). The growth indexes and physiological parameters of the seedlings of two strains were observed after 25 days of stress. And the accumulation of betaine and BT toxin protein. [results] the growth indexes of two lines were observed. The results showed that the growth of seedling height of transgenic poplar 107 was significantly higher than that of non-transgenic control under low salt concentration. Under high salt concentration, the height and diameter of two lines seedlings were significantly affected by salt stress. The physiological parameters of the two lines were observed under low salt concentration. The plasma membrane permeability of transgenic 107 poplar seedlings was significantly lower than that of the control, chlorophyll content increased to some extent, net photosynthetic rate and transpiration rate were significantly higher than that of the control, and the actual photosynthetic efficiency of light system 鈪，

本文编号：1808959