瘦素受体基因Gln223Arg多态性与西南地区汉族人群阻塞性睡眠呼吸暂停低通气综合征的关系

发布时间：2018-04-27 08:45

本文选题：阻塞性睡眠呼吸暂停低通气综合征 + 瘦素受体　；参考：《中国呼吸与危重监护杂志》2017年05期

【摘要】：目的探讨西南地区汉族人群瘦素受体基因Gln223Arg多态性与阻塞性睡眠呼吸暂停低通气综合征(OSAHS)之间的关系。方法选取西南地区汉族人群215例(其中OSAHS组116例,健康对照组99例);用聚合酶链反应(PCR)分析研究对象瘦素受体基因Gln223Arg多态性;双抗体酶联免疫吸附试验(ELISA)法测定瘦素(LEP)、真胰岛素(TI),同时测定体重指数(BMI)、颈围(NC)、腰围(WC)。结果 OSAHS组116例中,瘦素受体基因Gln223Arg多态性的GG、AA、GA的基因型频率表达分别为0.854、0.017、0.129,G等位基因频率为0.918;A等位基因频率为0.082。对照组中,瘦素受体基因Gln223Arg多态性的GG、AA、GA的基因型频率表达分别为0.840、0.020、0.14;G等位基因频率为0.90;A等位基因频率为0.10。两组基因型频率比较差异无统计学意义(X~2=0.784,P0.05)。Gln223Arg基因多态性的GG基因型OSAHS患者与(GA+AA)基因型OSAHS患者BMI、WC、NC比较差异有统计学意义(均P0.05)。对照组及轻、中、重度OSAHS组血清LEP、TI水平逐渐升高,各组间比较差异均有统计学意义(均P0.05)。结论瘦素受体基因型Gln223Arg多态性可能参与肥胖的发生,但与西南地区汉族人群OSAHS的发病无关;OSAHS患者Gln223Arg多态性与LEP、TI无关。OSAHS患者存在高瘦素血症、高胰岛素血症。
[Abstract]:Objective to investigate the relationship between leptin receptor gene Gln223Arg polymorphism and obstructive sleep apnea hypopnea syndrome (OSAHS). Methods the leptin receptor gene Gln223Arg polymorphism was analyzed by polymerase chain reaction (PCR) in 215 Han population in Southwest China (OSAHS group 116 cases and healthy control group 99 cases). The levels of leptin and insulin were determined by double antibody enzyme linked immunosorbent assay (Elisa), and the body mass index (BMI) was measured. Results in the OSAHS group, the genotypic frequencies of the Gln223Arg polymorphism of leptin receptor gene were 0.854, 0.017 and 0.129, respectively. The frequency of G allele was 0.918A and 0.082, respectively. In the control group, the genotypic frequencies of the Gln223Arg polymorphism of leptin receptor gene were 0.840 ~ 0.020 ~ (0.14) G allele and 0.90 ~ (0.90) A allele frequency, respectively. There was no significant difference in genotype frequency between the two groups. There was significant difference between the GG genotype OSAHS patients with GG genotype OSAHS and the GG genotype OSAHS patients (P 0.05). The level of serum LEP TI in the control group, mild, moderate and severe OSAHS group was increased gradually, and the difference was statistically significant among the groups (all P 0.05). Conclusion the Gln223Arg polymorphism of leptin receptor genotype may be involved in the development of obesity, but it is not related to the incidence of OSAHS in the Han nationality in Southwest China. The Gln223Arg polymorphism in OSAHS patients is not related to the leptin TI. There is hyperleptinemia and hyperinsulinemia in the patients with leptin receptor genotype.
【作者单位】：贵阳市第六人民医院急诊科;贵州省人民医院呼吸与危重医学科;
【基金】：贵州省科技支撑计划社会发展攻关项目(黔科合SY字[2009]3055)
【分类号】：R766

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