猪流行性腹泻病毒河南流行株S基因的克隆与遗传进化分析

发布时间：2018-04-27 10:04

本文选题：流行性腹泻病毒 + S基因　；参考：《中国兽医学报》2017年05期

【摘要】：近年来,我国大多省市暴发新生仔猪严重流行性腹泻,给养猪业造成巨大经济损失。为分析猪流行性腹泻病毒(PEDV)在河南省的流行和遗传变异情况,本研究对2014—2015年收集的30份临床腹泻样品,进行RT-PCR检测,对检测为阳性的PEDV样品进行S基因的克隆与序列分析。结果表明,30份临床腹泻样品中,RT-PCR检测出22个PEDV阳性样品,克隆的22条PEDV S序列与GenBank中发表的29条PEDVS基因序列核苷酸同源性为93.1%~99.2%。与经典毒株相比,22株河南流行株在S1区域存在碱基的插入、缺失现象;S基因遗传进化树表明,PEDV分为2群,河南流行株均属于PEDVП群,与近年国内分离株和韩国野毒株有较近亲缘关系,而与欧洲株以及中国目前使用的疫苗株亲缘关系较远,基因差异较大。
[Abstract]:In recent years, severe epidemic diarrhea of newborn piglets broke out in most provinces and cities of China, which caused huge economic losses to pig industry. In order to analyze the prevalence and genetic variation of porcine epidemic diarrhea virus (PEDVV) in Henan Province, 30 clinical diarrhea samples collected from 2014-2015 were detected by RT-PCR, and the S gene was cloned and sequenced from the positive PEDV samples. The results showed that 22 PEDV positive samples were detected by RT-PCR in 30 clinical diarrhea samples. The nucleotide homology of 22 PEDV S sequences and 29 PEDVS gene sequences published in GenBank was 93.1% 99.2%. Compared with the classical strains, 22 Henan epidemic strains had base insertions in the S1 region. The genetic evolution tree of the deletion of the S gene indicated that the PEDV was divided into two groups, and all of the Henan epidemic strains belonged to the PEDV group. There is a close relationship with the domestic isolates and Korean wild strains, but with the European strains and the vaccine strains currently used in China, the genetic differences are relatively large.
【作者单位】：河南农业大学牧医工程学院;河南牧业经济学院;郑州市猪重大疫病防控重点实验室;
【基金】：河南省产学研合作计划项目(152107000003)
【分类号】：S852.651

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