胁迫中华蜜蜂幼虫肠道的球囊菌及其体外培养的高表达基因分析

发布时间：2018-04-27 17:22

本文选题：中华蜜蜂 + RNA-seq　；参考：《福建农林大学学报(自然科学版)》2017年05期

【摘要】：利用RNA-seq技术对胁迫中华蜜蜂(简称中蜂)6日龄幼虫肠道的球囊菌及其体外培养进行深度测序,根据基因的FPKM值筛选得到高表达基因(HEGs),进而对HEGs进行GO及KEGG代谢通路(pathway)富集分析.Illumina测序数据经质控和过滤得到100814558条有效读段(clean reads),平均Q30均在93.81%以上.GO富集分析结果显示处理组(Aac T)的HEGs富集于39个GO term,基因富集数最多的是细胞(1872 unigene)、细胞组件(1872 unigene)和细胞进程(1748 unigenes);对照组(Aa CK)的HEGs富集于37个GO term,基因富集数最多的是细胞(785 unigenes),其次是细胞组件(785 unigenes)和代谢进程(776 unigenes).KEGG pathway富集分析显示,Aac T的HEGs富集在119个代谢通路上,基因富集数最多的是核糖体(176 unigenes)、碳代谢(147 unigenes)及氨基酸的生物合成(134 unigenes);Aa CK的HEGs富集在110个代谢通路上,基因富集数最多的是核糖体(179 unigenes)、氨基酸的生物合成(70 unigenes)以及碳代谢(62 unigenes).深入分析发现,对于富集在MAPK信号通路上的高表达基因数量,胁迫中蜂幼虫肠道的球囊菌远多于体外培养的球囊菌,说明病原的该通路在胁迫后期被显著激活.
[Abstract]:The RNA-seq technique was used to sequence the saccular bacteria of the intestinal tract of the 6-day-old larvae of honeybee (Apis cerana cerana cerana) under stress and its culture in vitro. According to the FPKM value of the gene, the highly expressed gene (HEGsN) was obtained, and then the HEGs was enriched by go and KEGG metabolic pathway. The sequence data of Illumina were analyzed by quality control and filtration. The average Q30 was more than 93.81%. Go enrichment analysis showed that the average Q30 was more than 93.81%. In fruit display treatment group, the HEGs of Aac T was enriched in 39 go terms.The most abundant number of genes was in the cell line 1872 unigenegene (cell component 1872 unigeneic) and the cell process 1 748 unigenesl. The HEGs of the control group was enriched in 37 go terms, and the most abundant gene was in the cell line 785 unigenesin. Secondly, the enrichment analysis of cell component 785 unigenes and metabolic process of 776 unigenes).KEGG pathway showed that the HEGs of Aac T was enriched in 119 metabolic pathways. The most abundant genes were ribosome 176unigenesi, carbon metabolism 147unigeneses) and amino acid biosynthesis (134unigenesus). The HEGs of Aa CK was enriched on 110 metabolic pathways, and the most abundant genes were ribosome 179unigenesi, amino acid biosynthesis 70 unigeneses) and carbon metabolism 62 unigenesus. It was found that the number of highly expressed genes enriched in the MAPK signaling pathway was much higher in the intestinal tract of the bee larvae under stress than that in the culture in vitro, indicating that the pathogenetic pathway was significantly activated at the later stage of stress.
【作者单位】：福建农林大学蜂学学院;
【基金】：现代农业产业技术体系建设专项资金(CARS-44-KXJ7) 福建省中青年教师教育科研项目(JAT17018) 福建农林大学科技发展基金(KF2015123) 国家级大学生创新创业项目(201610389016)
【分类号】：S895.137

【相似文献】