牦牛PLIN2基因的克隆及其在卵巢不同发情阶段的表达

发布时间：2018-04-27 23:31

本文选题：牦牛 + PLIN基因　；参考：《中国畜牧兽医》2017年03期

【摘要】：试验旨在研究牦牛PLIN2基因的序列特征和表达特性,并分析其表达与不同发情时期的卵巢的相关性。根据GenBank中已知的普通牛序列,设计克隆引物,经RT-PCR扩增得到牦牛PLIN2基因序列。RT-PCR检测PLIN2基因在各个组织中的表达量,实时荧光定量PCR分析卵巢不同发情时期的表达量。结果表明,牦牛PLIN2基因的编码区长为1 353bp,共编码450个氨基酸,为无跨膜结构的非分泌蛋白。与GenBank中已知的普通牛序列同源性达到99.4%,表明PLIN2基因在进化过程中相当保守。在牦牛的各个组织中PLIN2分布广泛,但不同组织中的表达有差异,卵巢、乳腺、胃和肾脏中相对较多,而肺脏中几乎没有表达。实时荧光定量PCR结果显示,在卵巢的不同发情时期PLIN2都有表达,且在黄体期的表达量最高,表明PLIN2基因在牦牛发情周期中起着不可忽视的作用。
[Abstract]:The aim of this study was to study the sequence and expression characteristics of yak PLIN2 gene, and to analyze the correlation between the expression of PLIN2 gene and ovary in different estrus. Based on the known common bovine sequences in GenBank, cloned primers were designed, and the yak PLIN2 gene sequence was amplified by RT-PCR. RT-PCR was used to detect the expression of PLIN2 gene in various tissues, and real-time fluorescence quantitative PCR was used to analyze the expression of PLIN2 gene in different estrus stages of ovary. The results showed that the coding region of yak PLIN2 gene was 1353 BP, encoding 450 amino acids, which was a non-secretory protein without transmembrane structure. The sequence homology of PLIN2 gene was 99.4 with the known common cattle sequence in GenBank, indicating that the PLIN2 gene was quite conserved in the course of evolution. PLIN2 was widely distributed in various tissues of yak, but the expression of PLIN2 was different in different tissues. The expression of PLIN2 in ovary, mammary gland, stomach and kidney was relatively high, but there was almost no expression in lung. The results of real-time fluorescence quantitative PCR showed that PLIN2 was expressed in different oestrus and the highest in luteal phase, indicating that PLIN2 gene plays an important role in yak estrus cycle.
【作者单位】：西南民族大学生命科学与技术学院;西南民族大学青藏高原研究院;
【基金】：四川省科技支撑计划(2014NZ0114) 西南民族大学研究生“创新型科研项目”(CX2016SZ094)
【分类号】：S823.85

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