中华蜜蜂气味受体基因AcerOr167的克隆及表达分析

发布时间：2018-04-29 11:57

本文选题：中华蜜蜂 + AcerOr　；参考：《环境昆虫学报》2017年01期

【摘要】：本研究通过RT-PCR获得中华蜜蜂气味受体基因Or167的cDNA序列,并采用多种生物信息学软件对其结构特征进行预测分析;利用荧光定量PCR检测Acer Or167 m RNA在工蜂羽化后不同发育阶段(1、5、10、15、20、25和30日龄)和不同组织(触角、头、胸、腹、足、翅)中的相对表达量。克隆获得中华蜜蜂Or167的c DNA序列,命名为Acer Or167(Gen Bank登录号为KF239369),其全长1311 bp,编码436个氨基酸,预测有5个跨膜结构域;多序列比对结果显示,中华蜜蜂Acer Or167与西方蜜蜂Amel Or167的一致性最高,达94%,而与毕氏粗角蚁Cbir Or4-like的一致性最低,为49%;荧光定量结果显示,Acer Or167 m RNA在成蜂不同发育期的各个组织中均有表达,且触角中的表达量极显著高于其它各个组织(P0.01),在头、胸、腹、足、翅中有少量表达;从触角不同发育阶段的表达情况来看,1日龄表达量最低,5日龄表达量显著升高,20日龄表达量最高,且极显著高于其它各日龄(P0.01)。推测Acer Or167可能在中华蜜蜂的嗅觉识别系统中起着重要的作用,与中华蜜蜂外出采集,识别花香气味有关。本研究为进一步深入研究中华蜜蜂传统气味受体的功能奠定理论基础。
[Abstract]:In this study, the cDNA sequence of the odor receptor gene Or167 of honeybee was obtained by RT-PCR, and its structural characteristics were predicted by various bioinformatics software. The relative expression of Acer Or167 m RNA in different tissues (antennae, head, chest, abdomen, feet and wings) and different tissues (antennae, head, chest, abdomen, feet and wings) were detected by fluorescence quantitative PCR. The c DNA sequence of honeybee Or167 was cloned, named Acer Or167(Gen Bank accession number KF239369, which is 1311 BP, encoding 436 amino acids, and predicted to have 5 transmembrane domains. The consistency between Acer Or167 and Amel Or167 was the highest, up to 94%, while the consistency with Cbir Or4-like was the lowest (49%). The fluorescence quantitative analysis showed that Acer Or167 m RNA was expressed in all tissues of adult bees at different developmental stages. The expression level in antennae was significantly higher than that in other tissues (P 0.01), and a small amount of expression was found in head, chest, abdomen, foot and wings. According to the expression of different developmental stages of antennae, the lowest expression at 1 day and the lowest at 5 days were the highest at 20 days of age, and extremely significantly higher than those of other days of age (P 0.01). It is speculated that Acer Or167 may play an important role in the olfactory recognition system of honeybee, which is related to the odour recognition of Chinese honeybee. This study provides a theoretical basis for further study on the function of traditional odor receptors in honeybees.
【作者单位】：山西农业大学动物科技学院;云南省农业科学院蚕桑蜜蜂研究所;山西农业大学生命科学学院;
【基金】：国家自然科学基金项目(31502021,31272513) 山西农业大学科技创新基金(2014012)
【分类号】：S891

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