象草CpCCoAOMT2基因的克隆及生物信息学分析

发布时间：2018-04-29 14:08

本文选题：象草 + 咖啡酰酸辅酶A-O-甲基转移酶　；参考：《江苏农业科学》2017年16期

【摘要】：从象草中克隆了CpCCoAOMT2基因,并对其进行了初步的生物信息学分析。利用转录组测序获得的表达序列信息,设计1对特异性的PCR引物,应用RT-PCR技术从象草中扩增出CpCCoAOMT2基因。对获得的基因进行序列同源性搜索、结构域搜索及3D结构预测、分子系统进化树构建等生物信息学分析。测序结果表明,CCoAOMT2基因全长926 bp,含110 bp的5'-UTR、741 bp的CDS和75 bp的3'-UTR,共编码246个氨基酸,Gen Bank登录号为Bank It1975804;Blastn和Blast T分析结果显示,CpCCoAOMT2基因及其编码的蛋白在核苷酸水平和氨基酸水平上与多种植物的CCoAOMT基因和CCoAOMT蛋白具同源性;预测CpCCoAOMT2蛋白相对分子质量为27 253,等电点为5.08,是一个不含跨膜结构域、不含信号肽分子的亲水性、稳定蛋白。分子进化树分析结果表明,CpCCoAOMT2蛋白与禾本科植物的CCoAOMT蛋白亲缘关系最近,与裸子植物和蕨类植物关系较近,而与双子叶植物的关系较远。
[Abstract]:CpCCoAOMT2 gene was cloned from elephant grass and analyzed with bioinformatics. A pair of specific PCR primers were designed based on the expression sequence information obtained by transcriptome sequencing. The CpCCoAOMT2 gene was amplified from elephant grass by RT-PCR technique. Sequence homology search, domain search, 3D structure prediction and molecular phylogenetic tree construction were used for bioinformatics analysis. The results of sequencing showed that CCoAOMT2 gene was 926 BP in length, containing 110bp CDS of 5- UTR741 BP and 75 BP of 3- UTR-UTR. it encode 246 amino acids Genin Bank as Bank It1975804 Blastn and Blast T analysis results show that CpCCoAOMT2 gene and its encoded protein are at nucleotide level and amino level. The acid level is homologous to the CCoAOMT gene and CCoAOMT protein of many plants. The relative molecular weight and isoelectric point of CpCCoAOMT2 protein were 27 253 and 5.08 respectively. It was a hydrophilic and stable protein without transmembrane domain and signal peptide. The results of molecular phylogenetic tree analysis showed that CpCCoAOMT2 protein was closely related to CCoAOMT protein of Gramineae, close to gymnosperms and pteridophytes, but far from dicotyledonous plants.
【作者单位】：江苏省农业科学院畜牧研究所;
【基金】：国家自然科学基金(编号:31302025) 江苏省农业科技自主创新资金[编号:CX(14)5035] 国家牧草产业技术体系盐城综合试验站项目(编号:CARS-35) 浙江省宁波市科技计划(编号:2016C10018)
【分类号】：Q943.2;S543.9

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