FABP3基因对心肌细胞凋亡的影响

发布时间：2018-04-30 15:57

本文选题：FABP + 心肌细胞　；参考：《中国老年学杂志》2017年19期

【摘要】：目的脂肪酸结合蛋白(FABP)3基因对心肌细胞凋亡的影响及机制。方法将H9C2心肌细胞分为空白对照组、阴性对照组、FABP3沉默组、FABP3沉默+空质粒组及FABP3沉默+过表达人FABP3组,转染72 h后Western印迹检测各组细胞中FABP3的蛋白表达;流式细胞仪检测细胞凋亡;Western印迹检测Cleaved caspase3、Bcl-2蛋白表达。结果空白对照组与阴性对照组及FABP3沉默组和FABP3沉默+空质粒组之间FABP3蛋白表达差异无统计学意义(P0.05),FABP3沉默组和FABP3沉默+空质粒组FABP3的蛋白表达显著低于空白对照组与阴性对照组,FABP3沉默+过表达人FABP3组FABP3的蛋白表达显著高于FABP3沉默组和FABP3沉默+空质粒组(P0.01);FABP3沉默组和FABP3沉默+空质粒组细胞凋亡率及Cleaved caspase3蛋白表达显著高于空白对照组与阴性对照组,Bcl-2蛋白表达显著低于空白对照组与阴性对照组FABP3(P0.01);沉默+过表达人FABP3组细胞凋亡率及Cleaved caspase3蛋白表达显著低于FABP3沉默组和FABP3沉默+空质粒组,Bcl-2蛋白表达显著高于FABP3沉默组和FABP3沉默+空质粒组(P0.01)。结论沉默FABP3的表达可促进H9C2心肌细胞凋亡,过表达则抑制细胞凋亡,其机制与调节Cleaved caspase3及Bcl-2蛋白表达有关。
[Abstract]:Objective to investigate the effect and mechanism of fatty acid binding protein (FABP3) gene on cardiomyocyte apoptosis. Methods H9C2 cardiomyocytes were divided into blank control group, negative control group and FABP3 silencing FABP3 group. The expression of FABP3 protein was detected by Western blot 72 h after transfection. Apoptosis was detected by flow cytometry. Western blot was used to detect the expression of Cleaved caspase3 and Bcl-2 protein. Results there was no significant difference in the expression of FABP3 protein between the blank control group and the negative control group, the FABP3 silencing group and the FABP3 silencing empty plasmid group. The expression of FABP3 protein in the blank control group and FABP3 silencing blank plasmid group was significantly lower than that in the blank control group. The expression of FABP3 protein in FABP3 group was significantly higher than that in FABP3 silencing group and FABP3 silencing empty plasmid group (P 0.01) and FABP3 silencing empty plasmid group. The apoptosis rate and Cleaved caspase3 protein expression in FABP3 silencing group and FABP3 silencing blank plasmid group were significantly higher than those in FABP3 silencing group and FABP3 silencing empty plasmid group. The expression of Bcl-2 protein in the control group and the negative control group was significantly lower than that in the blank control group and the negative control group, while the apoptosis rate and the expression of Cleaved caspase3 protein in the silent FABP3 group were significantly lower than those in the FABP3 silencing group and the FABP3 silencing empty plasmid group. White expression was significantly higher than that in FABP3 silencing group and FABP3 silencing empty plasmid group. Conclusion silencing the expression of FABP3 can promote the apoptosis of H9C2 cardiomyocytes, and over-expression can inhibit the apoptosis. The mechanism is related to the regulation of the expression of Cleaved caspase3 and Bcl-2 protein.
【作者单位】：华中科技大学同济医学院附属武汉中心医院网络医疗部;
【基金】：武汉市临床医学科研项目(WZ15D17)
【分类号】：R54

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