乳腺癌病变与Erp57和ERAP1基因表达水平的关系及其临床意义的研究

发布时间：2018-04-30 20:29

本文选题：乳腺肿瘤 + APM　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:观察女性乳腺癌中Erp57、ERAP1的表达情况,探讨其与乳腺癌各临床病理指标的关系。方法:收集新疆医科大学附属肿瘤医院石蜡包埋组织标本共148例(乳腺癌124例/纤维腺瘤24例),新鲜组织标本共48例(乳腺纤维瘤14例/乳腺癌34例),分别采用免疫组化SP法和实时荧光定量RT-PCR技术检测乳腺癌和纤维腺瘤组织中Erp57及ERAP1蛋白质和转录表达水平;采用χ2检验分析纤维腺瘤和乳腺癌中Erp57及ERAP1表达差异性;采用列联系数相关分析Erp57、ERAP1蛋白表达水平与乳腺癌病理特征的关系,采用T检验比较不同乳腺组织各基因转录表达水平。结果:(1)免疫组化显示:Erp57、ERAP1在乳腺癌中表达水平均低于纤维腺瘤组,分别为(58.8%/100%、47.5%/83.3%),各组之间具有显著差异(χ2=15.061,P0.01/χ2=10.328,P0.01);Erp57、ERAP1蛋白质表达与乳腺癌患者的病理分期、组织分级及腋窝淋巴结有关(P均0.05),此外,ERAP1与Ki67阳性表达率、年龄、三阴性有关(P均0.05)。(2)实时荧光定量RT-PCR显示:Erp57mRNA在纤维腺瘤中表达水平为(4.90±2.04),在乳腺癌中表达水平为(6.33±1.36),差异具有统计学意义(P0.05),ERAP1在纤维腺瘤中表达水平为(4.48±1.92),在乳腺癌中表达水平为(4.48±1.90),差异无统计学意义(P0.05)。结论:(1)Erp57、ERAP1蛋白表达水平下调可能是乳腺癌免疫逃逸机制之一,Erp57、ERAP1蛋白表达水平降低与乳腺癌临床病理特征具有相关性。(2)Erp57蛋白质表达水平降低和转录表达下调变化比较相应,然而ERAP1转录表达水平与乳腺纤维腺瘤比较无差异性改变。
[Abstract]:Objective: to observe the expression of ERAP1 in female breast cancer and to explore the relationship between the expression of ERAP1 and the clinicopathological indexes of breast cancer. Methods: 148 paraffin-embedded tissue specimens (124 breast cancer / 24 fibroadenoma) and 48 fresh tissue specimens (14 breast fibroma / 34 breast cancer) were collected from Cancer Hospital affiliated to Xinjiang Medical University. Immunohistochemical SP method and real-time fluorescence quantitative RT-PCR were used to detect the expression of Erp57 and ERAP1 protein and transcription in breast cancer and fibroadenoma. The difference of Erp57 and ERAP1 expression in fibroadenoma and breast cancer was analyzed by 蠂 2 test, the relationship between the expression level of Erp57 ERAP1 protein and the pathological characteristics of breast cancer was analyzed by column association number correlation, and the transcriptional expression levels of genes in different breast tissues were compared by T test. Results the expression level of Erp57 ERAP1 in breast cancer was significantly lower than that in fibroadenoma group, which was 58.8 / 100 and 47.57.5and / 83.3, respectively. There was a significant difference between the two groups (蠂 ~ 2 ~ (15) 061n P 0.01 / 蠂 ~ (2) 10.328P ~ (0.011) ERAP1 protein expression and the pathological stage of breast cancer patients. The histological grade and axillary lymph node related P were 0.05%, in addition, the positive expression rate of ERAP1 and Ki67, age, and age, The expression level of Erp57 mRNA in fibroadenoma and breast cancer was 4.90 卤2.04 and 6.33 卤1.36, respectively. The difference was statistically significant (P < 0.05). The expression level of ERAP1 in fibroadenoma was 4.48 卤1.922, and that in breast cancer was 4.48 卤1.922. The level was 4.48 卤1.90, and the difference was not statistically significant (P 0.05). Conclusion the down-regulation of Erp57 ERAP1 protein expression level may be one of the mechanisms of immune escape in breast cancer. The decrease of ERAP1 protein expression level is related to the clinicopathological features of breast cancer. The decrease of Erp57 protein expression level and the down-regulation of transcription expression are related to the clinicopathological characteristics of breast cancer. However, there was no difference between ERAP1 transcription and breast fibroadenoma.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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