6株猪流行性腹泻病毒贵州株S基因序列分析

发布时间：2018-05-01 06:38

本文选题：猪流行性腹泻 + S基因　；参考：《中国畜牧兽医》2017年06期

【摘要】：为了解贵州省猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)S基因的特点,掌握其遗传变异情况,本试验设计3对特异性引物对6株PEDV贵州株S基因进行全基因RT-PCR扩增、克隆及序列测定;应用生物信息学软件将6株PEDV贵州流行株与参考毒株进行同源性与系统进化分析。结果显示,6株PEDV贵州流行株S基因的全基因长为4 161bp,编码1 387个氨基酸,与国内外PEDV参考毒株核苷酸同源性在93.3%~98.8%之间,氨基酸同源性在91.6%~98.9%之间;进化树分析结果显示,6株流行毒株与美国毒株、越南毒株和山东毒株亲缘关系较近;与CV777株、LZC、Brl、83P-5亲缘关系较远。结果表明,PEDV贵州地方流行毒株S基因编码的氨基酸存在一定程度变化,近年来呈现变异趋势。本研究可为贵州省PEDV的防控提供一定的理论依据。
[Abstract]:In order to understand the characteristics and genetic variation of porcine epidemic diarrhea virus (PEDVS) gene of porcine epidemic diarrhea virus in Guizhou Province, three pairs of specific primers were designed to amplify, clone and sequence the S gene of 6 strains of PEDV Guizhou strain. Bioinformatics software was used to analyze the homology and phylogenetic analysis of 6 PEDV epidemic strains and reference strains. The results showed that the total length of S gene was 4 161 BP, encoding 1 387 amino acids. The nucleotide homology of S gene was 93. 398. 8% and amino acid homology was 91.6%. The results of phylogenetic tree analysis showed that 6 epidemic strains were closely related to the American strain, Vietnam strain and Shandong strain, and far related to the CV777 strain LZCCnBrlC83P-5. The results of phylogenetic tree analysis showed that the 6 strains were closely related to the American strain, the Vietnam strain and the Shandong strain. The results showed that the amino acids encoded by S gene of PEDV endemic strain in Guizhou were changed to a certain extent and showed a trend of variation in recent years. This study can provide some theoretical basis for the prevention and control of PEDV in Guizhou province.
【作者单位】：贵州省贵阳市花溪区农业局;贵州省动物疫病预防控制中心;贵州大学动物科学学院;
【基金】：贵州省科学技术厅农业攻关项目“贵州省仔猪腹泻性疫病风险分析及综合防控技术研究”(黔科合NY[2015]3009-1号) 贵州省生猪质量安全工程技术研究中心建设项目“生猪健康养殖实验室建设与技术研发”黔科合农C字[2011]4022号贵州省农业委员会科技计划项“2015年贵州省重大动物疫病省级定点监测”[2015-11号]
【分类号】：S852.651

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