紧密连接蛋白claudin-6基因表达与结肠癌关系的研究

发布时间：2018-05-01 19:33

本文选题：结肠癌 + claudin-基因　；参考：《现代预防医学》2017年16期

【摘要】：目的观察claudin-6基因在结肠癌、结肠腺瘤及正常结肠黏膜组织中的表达,以探讨其与结肠癌发生发展的关系。方法应用原位杂交法及免疫组织化学法对claudin-6 mRNA及其蛋白在结肠腺瘤(n=20)、结肠癌(n=42)及正常结肠组织(n=10)中的表达进行检测。结果 claudin-6 mRNA及其蛋白在结肠腺瘤及结肠癌组织中的阳性表达率低于结肠正常黏膜,二者在结肠癌组织中的表达又低于结肠腺瘤,组间表达差异有统计学意义(χ~2=9.235,P0.05;χ~2=8.352,P0.05);claudin-6 mRNA及其蛋白的表达均与结肠癌组织的分化程度高低、有无淋巴结的转移及浸润肠壁的深度有关(P0.05),二者在年龄、肿瘤大小及性别组内的表达差异无统计学意义(P0.05)。结论 claudin-6基因在结肠癌组织中呈低表达,提示claudin-6表达的下调与结肠癌的发生、发展有密切关系。
[Abstract]:Objective to investigate the expression of claudin-6 gene in colon cancer, colon adenoma and normal colonic mucosa. Methods the expression of claudin-6 mRNA and its protein were detected by in situ hybridization and immunohistochemistry in adenoma of colon, adenoma nongma (42) and normal colonic tissue (10). Results the positive expression rate of claudin-6 mRNA and its protein in colon adenoma and colon cancer tissue was lower than that in colon normal mucosa, and the expression of claudin-6 mRNA and protein in colon cancer tissue was lower than that in colon adenoma. The expression of claudin-6 mRNA and its protein were correlated with the differentiation of colon cancer tissue, lymph node metastasis and the depth of invasion of intestinal wall (P 0.05). The expression of claudin-6 mRNA and its protein were related to the age of colonic carcinoma, and the expression of claudin-6 mRNA and its protein were related to the degree of differentiation, lymph node metastasis and the depth of invasion of intestinal wall. There was no significant difference in tumor size and expression in sex group (P 0.05). Conclusion the low expression of claudin-6 gene in colon cancer tissues suggests that the down-regulation of claudin-6 expression is closely related to the occurrence and development of colon cancer.
【作者单位】：邢台医学高等专科学校病理教研室;邢台医学高等专科学校第二附属医院病理科;
【分类号】：R735.35

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