叶酸代谢相关酶系基因多态性与不良孕史的相关性研究

发布时间：2018-05-01 22:42

本文选题：亚甲基四氢叶酸还原酶基因 + 甲硫氨酸合成酶还原酶基因　；参考：《中国妇产科临床杂志》2017年04期

【摘要】：目的探讨亚甲基四氢叶酸还原酶(MTHFR)、甲硫氨酸合成酶还原酶(MTRR)、还原叶酸载体(RFC-1)及蛋氨酸合酶(MTR)等叶酸代谢酶系基因多态性与不良孕史的关系。方法收集2012年3月至2015年4月本院未孕女性213例,根据既往有无不良孕史分为不良孕史组65例(研究组)和无不良孕史组148例(对照组)。采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测MTHFR、MTRR、RFC-1及MTR基因分布频率,根据各基因位点功能、纯合、杂合出现频率将叶酸利用能力分为正常、略差、差、很差四个等级,比较基因多态性与不良孕史的关系及两组叶酸利用能力分布差异。结果携带MTHFR TT基因型的母亲既往不良孕史风险高于MTHFR CC+CT基因型(OR=3.235,95%CI:1.223~10.215,P0.05),携带MTRR GG基因型的母亲既往不良孕史风险同样高于AA+AG基因型(OR=3.982,95%CI:1.631~9.990,P0.05);而RFC-1 AA与AG+GG基因型、MTR GG与AA+AG基因型既往不良孕史风险比较,差异(P0.05);研究组叶酸利用能力正常、略差率分别为1.54%、20.00%,低于对照组的33.78%和41.22%,而差和很差率分别为44.62%和33.85%,高于对照组的13.51%和1.49%(P0.05)。结论母亲携带MTHFR 677CT基因型、MTR66AG基因型与不良孕史有关,而RFC-1 80GA基因型,MTR 2756AG基因型与不良孕史无显著相关性。
[Abstract]:Objective to investigate the relationship between methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), reductive folate carrier (RFC-1) and methionine synthase (MTR). Methods 213 non-pregnant women from March 2012 to April 2015 in our hospital were divided into two groups according to the history of unfavorable pregnancy: 65 cases (study group) and 148 cases (control group). Polymerase chain reaction-restriction fragment length polymorphism (RFRFLP) method was used to detect the distribution frequency of MTHFRRRRRC-1 and MTR genes. According to the function of each locus, homozygosity and heterozygosity, folic acid utilization was divided into normal and slightly poor. The relationship between gene polymorphism and history of poor pregnancy and the distribution of folic acid utilization ability between the two groups were compared. Results the risk of adverse pregnancy history of mothers with MTHFR TT genotype was higher than that of MTHFR CC CT genotype ORG 3.235 / 95, and that of mothers with MTRR GG genotype was also higher than that of MTHFR CC CT AG genotype OR3.982 / 95CI1.6319.990P0.055.The ratio of RFC-1 AA and AG GG genotypes was MTRGG and AA. Comparing the risk of AG genotype with previous adverse pregnancy history, The difference of folic acid utilization capacity in the study group was normal, and the difference rate was 1.54% and 20.00, which was lower than that in the control group (33.78% and 41.22%), while the difference and very difference rates were 44.62% and 33.85%, respectively, which were higher than those in the control group (13.51%) and 1.49% (P 0.05). Conclusion the mother carrying MTHFR 677CT genotype / MTR66AG genotype is associated with the history of poor pregnancy, while the RFC-1 80GA genotype / MTR66AG genotype has no significant correlation with the bad pregnancy history.
【作者单位】：鄂东医疗黄石市中心医院生殖中心湖北理工学院附属医院;
【分类号】：R714.2

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