APEC感染雏鸡小肠炎症相关基因的RNA-Seq分析

发布时间：2018-05-02 07:26

本文选题：禽致病性大肠杆菌 + 雏鸡小肠　；参考：《西北农林科技大学学报(自然科学版)》2017年08期

【摘要】：【目的】研究禽致病性大肠杆菌(APEC)感染雏鸡小肠炎症相关基因表达的变化。【方法】对14日龄雏鸡腿部肌肉注射0.5mL/羽1×106 CFU/mL的APEC菌液,观察雏鸡的发病情况,并取病变严重雏鸡空肠为测序样品,同时以注射等量生理盐水为对照,采用RNA高通量测序(RNA-Seq)技术,筛选分析感染和未感染APEC雏鸡小肠炎症相关基因表达的变化。【结果】以差异倍数在2倍及以上为标准,从试验组与对照组共筛选出差异表达基因131个。GO分类结果显示,有87个差异基因得到491个GO功能注释,这些基因主要富集在炎症反应、肝素整合、白细胞介素-6的生物合成等过程。基于KEGG分析,仅有29个差异基因得到注释,涉及41个信号通路,主要参与的信号通路有PPAR、花生四烯酸代谢、MAPK等。【结论】APEC感染雏鸡后会引起机体炎症相关基因表达的变化,从中筛选出10个可能在炎症反应中具有重要影响的炎症相关基因。
[Abstract]:[objective] to study the expression changes of inflammatory genes related to intestinal inflammation in chickens infected with avian pathogenic Escherichia coli (0.5mL/). [methods] to observe the incidence of 0.5mL/ plume (1 脳 10 6 CFU/mL) by intramuscular injection of 1 脳 10 6 CFU/mL APEC bacteria into the legs of 14 day old chicks. The jejunum of severely diseased chicks was taken as sequencing sample, and the same amount of saline was injected as control. The RNA high-throughput sequencing technique was used. The expression of inflammatory genes in small intestine of infected and uninfected APEC chicks was analyzed by screening and analyzing. [results] based on the standard of 2 times and more than 2 times of difference, 131 differentially expressed genes were selected from the experimental group and the control group. There are 87 differentially expressed genes with 491 go functional annotations. These genes are mainly enriched in inflammatory reaction, heparin integration, interleukin-6 biosynthesis and so on. Based on KEGG analysis, only 29 differentially expressed genes were annotated, involving 41 signaling pathways, mainly involved in PPAR, arachidonic acid metabolism and MAPK.Conclusion APEC infection may cause changes in the expression of inflammatory genes in chickens. Ten inflammatory genes that may play an important role in inflammatory response were screened.
【作者单位】：安徽农业大学动物科技学院;
【基金】：国家自然科学基金项目(31372402);国家自然科学基金青年项目(31502038)
【分类号】：S858.31

【相似文献】