HBx基因诱导肝卵圆细胞恶性转化并促进其发生侵袭转移

发布时间：2018-05-02 12:10

本文选题：卵圆细胞 + HBx　；参考：《华中科技大学学报(医学版)》2017年04期

【摘要】：目的研究HBx对肝卵圆细胞发生恶性转化并上皮-间质转化(EMT)的影响。方法以稳定表达HBx的转基因卵圆细胞株pEGFP-HBx-LE/6为实验组,pEGFP-LE/6卵圆细胞为对照组。使用Western blotting、Transwell细胞侵袭实验、划痕实验、CCK-8等检测pEGFP-HBx-LE/6在细胞周期、分化标志物以及增殖转移能力方面的变化。结果(1)实验组细胞体积增大、具有明显异形性,CK-19、CK-7表达明显下降,AFP、HNF-4α表达升高;(2)实验组细胞的增殖能力明显升高,克隆形成能力、侵袭能力、划痕恢复能力明显增强;(3)与对照组比较,实验组E-cadherin表达水平明显下降,而N-cadherin、α-SMA、Vimentin表达水平明显上升。结论 HBx基因可诱导肝卵圆细胞向恶性转化,并促进其转移。
[Abstract]:Objective to study the effect of HBx on malignant transformation and epithelial-interstitial transformation of hepatic oval cells. Methods pEGFP-HBx-LE/6, a transgenic oval cell line expressing HBx stably, was used as experimental group and pEGFP-LER / 6 oval cells as control group. The changes of cell cycle, differentiation markers and proliferation and metastasis ability of pEGFP-HBx-LE/6 were detected by Western blottingTranswell cell invasion assay and scratching test (CCK-8). Results 1) the cell volume of the experimental group increased, and the expression of CK-19CK-7 decreased significantly. The expression of AFPnF-4 伪 increased significantly. The proliferation ability, clone formation ability and invasion ability of the experimental group cells were significantly increased. Compared with the control group, the expression of E-cadherin in the experimental group decreased significantly, while the expression of N-cadherin, 伪 -SMA-Vimentin increased significantly. Conclusion HBx gene can induce the malignant transformation of hepatic oval cells and promote its metastasis.
【作者单位】：华中科技大学同济医学院附属同济医院肿瘤科;华中科技大学同济医学院附属同济医院肝脏外科;
【基金】：国家自然科学基金青年基金资助项目(No.81402410,No.81602626)
【分类号】：R735.7

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