烧伤创面感染与金黄色葡萄球菌毒力基因相关分析

发布时间：2018-05-02 18:02

本文选题：烧伤创面感染 + 金黄色葡萄球菌　；参考：《中国病原生物学杂志》2017年04期

【摘要】：目的通过分析烧伤创面感染与金黄色葡萄球菌毒力基因的相关性,为临床合理用药提供指导。方法收集河北医科大学第一医院烧伤创面感染患者送检标本分离金黄色葡萄球菌。PCR扩增毒力基因,并采用统计学分析烧伤创面感染与金黄色葡萄球菌毒力基因的相关性。结果分离出149株金黄色葡萄球菌,其中分离自痰液95株,占63.76%;脓液21株,占14.09%;血液16株,占10.74%;分泌物11株,占7.38%;其他标本6株,占4.03%。PCR扩增检测细菌毒力基因,sasX、psm-mec、pvl毒力基因大小分别为615、210和156bp,检出率分别为8.05%、2.82%和54.36%。分析烧伤创面感染与金黄色葡萄球菌毒力基因sasX、psm-mec和pvl的相关性,OR值分别为0.8147(95%CI为0.2464~2.6935)、2.2548(95%CI为1.0287~4.9422)和0.3042(95%CI为0.1551~0.5966)。结论金黄色葡萄球菌毒力基因psm-mec与烧伤患者创面感染程度有关,与sasX和pvl无关。
[Abstract]:Objective to analyze the relationship between the infection of burn wound and the virulence gene of Staphylococcus aureus. Methods Staphylococcus aureus and Staphylococcus aureus virulence gene were isolated from burn wound infected patients in the first Hospital of Hebei Medical University. The relationship between the infection of burn wound and the virulence gene of Staphylococcus aureus was analyzed statistically. Results 149 strains of Staphylococcus aureus were isolated, of which 95 were isolated from sputum (63.76), 21 from pus (14.09), 16 from blood (10.74), 11 from secretions (7.38), and 6 from other samples. The size of virulence gene of bacterial virulence gene psm-mecl detected by 4.03%.PCR amplification was 615210 and 156bprespectively, and the detection rate was 8.05% and 54.36%, respectively. To analyze the correlation between burn wound infection and staphylococcus aureus virulence gene sasXnpsm-mec and pvl, OR values were 0.2464, 2.6935 and 2.2548, 95 CI were 1.02874.9422 and 0.3042(95%CI were 0.1551 and 0.5966respectively. Conclusion Staphylococcus aureus virulence gene psm-mec is related to the degree of wound infection in burn patients, but not to sasX and pvl.
【作者单位】：张家口学院护理学院;河北医科大学第一医院;
【分类号】：R644

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