马尾松响应松材线虫侵染的基因动态表达变化

发布时间：2018-05-03 13:55

本文选题：马尾松 + 松材线虫　；参考：《福建农业学报》2017年04期

【摘要】：松材线虫病是一种严重危害马尾松生长的流行性病害,可导致马尾松枯萎死亡。为揭示该过程中基因的表达变化行为,本研究利用荧光定量PCR技术检测了松材线虫侵染不同天数下的马尾松较其对照样本中病原识别、抗逆调节、次生代谢、解毒作用及生长素响应等相关基因的表达变化。结果显示,除了与病原识别相关的CC-NBS-LRR抗性蛋白基因的表达随侵染天数的增加而增强之外,其他基因则在侵染2d的马尾松样本中的表达水平最高,且明显高于未受侵染的对照马尾松样本,随后在侵染3天的马尾松中的表达又低于对照样本。此外,黄酮-3-羟化酶和细胞色素P450基因的表达随着侵染虫量的增加呈先上调后下调的变化方式。通过本研究初步揭示了马尾松响应松材线虫侵染的基因表达变化模式。
[Abstract]:Pine wood nematode disease is an epidemic disease that seriously damages the growth of Pinus massoniana and can lead to the wilting and death of Pinus massoniana. In order to reveal the change of gene expression during this process, the pathogen identification, stress tolerance regulation and secondary metabolism of Pinus massoniana were detected by fluorescence quantitative PCR under different days of infection by pine wood nematode. Detoxification and auxin response and other related gene expression changes. The results showed that the expression of CC-NBS-LRR resistance protein gene was increased with the increase of infection days, but the expression level of other genes was the highest in Pinus massoniana samples infected for 2 days. The expression level of Pinus massoniana was significantly higher than that of uninfected Pinus massoniana, and then the expression of Pinus massoniana after 3 days of infection was lower than that of the control. In addition, the expression of flavonoids -3-hydroxylase and cytochrome P450 genes increased with the increase of the number of infecting insects. The gene expression pattern of Pinus massoniana in response to pine wood nematode infection was preliminarily revealed.
【作者单位】：福建农林大学林学院;福建农林大学金山学院;
【基金】：福建省财政厅资助项目(K81139238,K8911010)
【分类号】：S763.7

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