过表达GmATG8c基因提高大豆的低氮耐受性和产量

发布时间：2018-05-03 16:59

  本文选题：大豆 + 细胞自噬　； 参考：《植物生理学报》2017年02期

【摘要】：大豆是一种高需氮素作物,其籽粒发育所需氮素在很大程度上来源于"源"器官中氮素的再动员,而细胞自噬是植物进行氮素再动员的主要形式之一。为了研究大豆细胞自噬关键基因GmATG8c的功能,本文利用35S启动子驱动GmATG8c基因在大豆中过表达。通过农杆菌介导的大豆子叶节转化法获得了3个独立的单拷贝35S:GmATG8c转基因大豆株系,半定量RT-PCR结果显示,所有转基因株系中目的基因均得到了高效表达。进一步对转基因大豆株系进行的低氮耐受性和产量相关性状分析结果表明,在低氮培养条件下,35S:GmATG8c转基因大豆的生长速率加快,二级分枝数和单株粒数增多,百粒重增加,单株产量显著提高。说明GmATG8c是一个具有提高NUE(nitrogen use effi ciency)育种潜力的基因。
[Abstract]:Soybean is a kind of high nitrogen demand crop. The nitrogen needed for grain development of soybean comes from nitrogen remobilization in "source" organs to a great extent, and cellular autophagy is one of the main forms of nitrogen remobilization in plants. In order to study the function of key gene GmATG8c in soybean cell autophagy, 35s promoter was used to drive the overexpression of GmATG8c gene in soybean. Three independent single-copy 35S:GmATG8c transgenic soybean lines were obtained by Agrobacterium tumefaciens mediated cotyledon node transformation. The semi-quantitative RT-PCR results showed that the target genes were highly expressed in all transgenic lines. The results of analysis on low nitrogen tolerance and yield related characters of transgenic soybean lines showed that under low nitrogen culture, the growth rate of transgenic soybean was accelerated, the number of secondary branches and grains per plant increased, and the weight of 100 seeds increased. The yield per plant increased significantly. It is suggested that GmATG8c is a gene with the potential to improve NUE(nitrogen use effi breeding.
【作者单位】： 南开大学生命科学学院;
【基金】：国家转基因生物新品种培育科技重大专项(2016ZX0800-4005-004和2014Z X0800930B-002) 国家自然科学基金(31370285)~~
【分类号】：S565.1
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本文编号：1839323