花生中三个LEA基因的克隆与表达分析

发布时间：2018-05-03 18:39

本文选题：花生 + 胚胎发生晚期丰富蛋白　；参考：《花生学报》2016年04期

【摘要】：胚胎发生晚期丰富蛋白(LEA)是一类受发育阶段及脱水信号调节的脱水保护蛋白,在响应植物干旱、低温、高盐等逆境胁迫中具有重要功能。本研究在转录组数据分析时,发现3个LEA基因在花生干旱胁迫时大量上调表达。利用RACE技术以及生物信息学方法,发现这三个基因分别属于LEA2、LEA3以及LEA4。通过序列比对进行进化分析,结果表明其均与Arachis duranensis,A.ipaensis相关基因具有极高相似度,印证了栽培花生来源于Arachis duranensis和A.ipaensis的研究结果。通过表达分析发现,正常状态下这三个基因在花生根、茎、叶中均有表达;干旱胁迫状态下,花生根部的LEA基因大量表达,暗示其在花生抗旱机制中发挥重要作用。本研究结果可为筛选新的抗旱花生种质提供理论依据。
[Abstract]:Lea is a kind of dehydration protection protein regulated by developmental stage and dehydration signal, which plays an important role in response to drought, low temperature and high salt stress. In this study, we found that three LEA genes were upregulated under drought stress in peanut. By using RACE technique and bioinformatics method, we found that these three genes belong to LEA2LEA3 and LEA4, respectively. The results of sequence alignment analysis showed that both of them had very high similarity with Arachis duranensis A. ipaensis related genes, which confirmed the results of the cultivation of peanut derived from Arachis duranensis and A.ipaensis. It was found that these three genes were expressed in peanut roots, stems and leaves under normal condition, and that LEA genes in peanut roots were highly expressed under drought stress, suggesting that they played an important role in peanut drought resistance mechanism. The results of this study can provide theoretical basis for screening new drought resistant peanut germplasm.
【作者单位】：山东省花生研究所;山东农业大学农学院;
【基金】：山东省农科院青年基金(2016YQN16) 国家国际科技合作专项(2015DFA31190) 山东省现代农业产业技术体系花生遗传育种岗位(SDAIT-04-02) 国家科技支撑计划(2014BAD11B04) 花生抗逆、广适种质资源创新与利用(山东省农业良种工程)
【分类号】：S565.2

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