NaClO胁迫下单核细胞增生李斯特菌WaX12及其sigB基因缺失突变株抗氧化胁迫相关功能基因表达比较

发布时间：2018-05-03 22:44

  本文选题：单增李斯特菌 + sigB　； 参考：《食品科学》2017年10期

【摘要】：目的:探究单核细胞增生李斯特菌sigB基因对次氯酸钠(NaClO)胁迫的调控作用。方法:在半致死浓度NaClO(3 mmol/L)胁迫下,比较该菌野生株WaX12与sigB基因敲除突变株的耐受程度及菌内的活性氧水平,并通过实时定量聚合酶链式反应技术(real-time quantitative polymerase chain reaction,RT-PCR)进一步观察lmo1433、lmo0906、lmo2344、ohrR、lmo2770基因随胁迫时间的转录水平变化。结果:野生株和sigB突变株的表型测定结果显示,野生株比缺失株更耐NaClO胁迫,而且此现象在稳定期更加明显;当胁迫时间达到30 min时,突变株内的活性氧水平增长率比野生株高出19.97%,进一步说明sigB基因对NaClO胁迫有一定的调控作用。RT-PCR结果表明,基因lmo1433、lmo2344和ohrR在野生株的表达水平显著高于突变株(P0.01),而基因lmo0906、lmo2770的表达水平在野生株和缺失株中的差异并不明显。结论:研究表明,单核细胞增生李斯特菌sigB基因对NaClO胁迫有一定的影响作用,且部分作用可能由激活部分调节机体氧化还原平衡的基因得以实现。
[Abstract]:Aim: to investigate the effect of sigB gene of Listeria monocytogenes on sodium hypochlorite (NaClO) stress. Methods: under the stress of NaClO(3 mmol / L, the tolerance of WaX12 and sigB knockout mutant and the level of reactive oxygen species in the wild strain were compared. The real-time quantitative polymerase chain reactionation (RT-PCR) technique was used to further observe the transcriptional level of lmo1433nlmo0906 / lmo234ohrRlmo2770 gene under stress time. Results: the phenotypic analysis of wild and sigB mutants showed that wild plants were more resistant to NaClO stress than the absent strains, and this phenomenon was more obvious in the stable period, and when the stress time reached 30 min, The growth rate of Ros in the mutant was 19.97 higher than that in the wild plant, which further indicated that sigB gene could regulate NaClO stress. RT-PCR results showed that the Ros level of the mutant was higher than that of the wild strain. The expression levels of lmo1433, lmo2344 and ohrR in wild plants were significantly higher than those in mutant strain P0.01, but the expression level of gene lmo0906 and lmo2770 was not significantly different between wild and absent plants. Conclusion: the results suggest that sigB gene of Listeria monocytogenes has a certain effect on NaClO stress, and part of the effect may be realized by activating genes that regulate the redox balance.
【作者单位】： 上海海洋大学食品学院农业部水产品贮藏保鲜质量安全风险评估实验室水产品加工及贮藏工程技术研究中心;
【基金】：国家自然科学基金面上项目(31271870;31571917) 上海市科委计划项目(14DZ1205100;14320502100);上海市科委工程中心建设项目(11DZ2280300) 上海市科技兴农重点攻关项目(沪农科攻字2014第3-5号;2015第4-8号)
【分类号】：Q933

【相似文献】

相关期刊论文 前1条

1 刘会娟;范丽微;王晓萍;;NaClO对黑藻叶片细胞质流动的影响[J];哈尔滨师范大学自然科学学报;2008年02期

，

本文编号：1840443