茶树Copine家族基因CsBON3的克隆与表达分析

发布时间：2018-05-04 02:51

  本文选题：茶树 + Copine家族　； 参考：《茶叶科学》2017年06期

【摘要】：Copine蛋白是一类包含2个C2(N端)和1个vWA(C端)保守域的Ca~(2+)依赖蛋白或磷脂结合蛋白,在胞内信号转导中发挥重要作用。基于序列相似性分析,从茶树转录组数据库中筛选出1条与Copine家族基因高度同源的EST序列。经测序验证该序列包含1 746 bp的完整ORF,编码581个氨基酸。同源比对显示该基因与拟南芥At BON3序列相似度最高(65%),将其命名为CsBON3(Gen Bank登录号为KY435900)。生物信息学分析显示,CsBON3蛋白分子量为63.66 k D,理论等电点为5.48;具有Copine家族蛋白特有的保守结构域;属亲水性蛋白,无信号肽位点,非分泌性蛋白,无跨膜结构域。表达分析表明,CsBON3在茶树花和根系中表达量最高,茎干和成熟叶中表达量最低。低温(4℃)处理茶树1 d后,其表达被显著上调;在生长阶段,该基因表达量高于休眠阶段;同时在接种炭疽菌的茶树叶片中,该基因也被快速上调,表明该基因可能与茶树低温、生长发育及抗病相关。
[Abstract]:Copine proteins are a class of Ca~(2 / phospholipid binding proteins that contain two C2N terminal and one vWA(C conserved domain and play an important role in intracellular signal transduction. Based on sequence similarity analysis, a EST sequence with high homology to Copine family genes was screened from tea transcriptional database. The sequence was confirmed to contain 1 746 BP complete ORF encoding 581 amino acids. Homologous alignment showed that the gene had the highest similarity with Arabidopsis thaliana at BON3 sequence, and it was named CsBON3(Gen Bank accession number KY435900. Bioinformatics analysis showed that the molecular weight of CsBON3 protein was 63.66kD, and the theoretical isoelectric point was 5.48. it had the conserved domain of Copine family protein, which belonged to hydrophilic protein, no signal peptide site, non-secretory protein and no transmembrane domain. The expression of CsBON3 was the highest in tea flower and root system, and the lowest in stem and mature leaf. The expression of the gene was significantly up-regulated in tea plants treated at 4 鈩，

本文编号：1841265