支气管哮喘患儿CHI3L1基因多态性与气道重塑及炎症反应关联性的研究

发布时间：2018-05-04 22:34

本文选题：支气管哮喘 + CHI3L1基因多态性　；参考：《兰州大学》2017年硕士论文

【摘要】：目的:研究哮喘患儿易感基因CHI3L1多态性与气道重塑及炎症反应的关联性。方法:选取2014年6月至2016年3月在咸阳市第一人民医院儿科确诊为轻度支气管哮喘的68例患儿作为哮喘组,同期体检的52例健康儿童作为对照组。提取外周血基因组DNA,采用Mass ARRAY-IPLEX质谱阵列法测定CHI3LI基因的rs4950928、rs883125及rs10399805位点的多态性,采用ELISA法测定气道重塑指标、炎症反应指标的含量。结果:1哮喘组CHI3L1基因rs10399805位点基因型TT、TC、CC以及rs883125位点基因型CC、GC、GG的基因频率与对照组比较无统计学意义(P0.05)。2哮喘组CHI3L1基因rs4950928位点基因型GG、GC的基因频率高于对照组、基因型CC的基因频率低于对照组,差异均有统计学意义(P0.05)。3哮喘组CHI3L1基因rs4950928位点GC基因型、GG基因型血清中TGF-β1、Act-A、OPN、Gal-3、Ang II、IL-17、IL-22的含量均高于CC基因型,差异均有统计学意义(P0.05)。(4)哮喘组CHI3L1基因rs4950928位点GC基因型、GG基因型血清中IL-10、IL-12的含量均低于CC基因型,两组比较差异有统计学意义(P0.05)。(5)哮喘组CHI3L1基因rs4950928位点GC基因型血清中TGF-β1、Act-A、OPN、Gal-3、Ang II、IL-10、IL-12、IL-17、IL-22的含量与GG基因型比较差异无统计学意义(P0.05)。结论:CHI3L1基因rs4950928位点多态性可能为儿童支气管哮喘的易感因子;rs4950928位点基因型GC、GG可能会加重哮喘患儿气道重塑及炎症反应。
[Abstract]:Objective: to study the association of susceptibility gene CHI3L1 polymorphism with airway remodeling and inflammation in asthmatic children. Methods: from June 2014 to March 2016, 68 children with mild bronchial asthma were selected as asthma group and 52 healthy children as control group. The genomic DNA of peripheral blood was extracted and the polymorphism of rs4950928 rs883125 and rs10399805 of CHI3LI gene was determined by Mass ARRAY-IPLEX mass spectrometry. The airway remodeling index and the content of inflammatory response index were measured by ELISA method. Results compared with the control group, there was no significant difference in the frequency of the rs10399805 genotype of CHI3L1 gene, the genotype of rs10399805 locus, and the gene frequency of the rs883125 locus genotype of rs883125 gene. The frequency of CHI3L1 gene rs4950928 locus GGG gene in the asthma group was significantly higher than that in the control group, compared with that in the control group. The frequency of CC gene was significantly lower than that of the control group, and the levels of TGF- 尾 1, Act-AOPNN Gal-3Ang III-IL-17 and IL-22 in the serum of rs4950928 locus of CHI3L1 gene in asthmatic group were higher than those in CC genotype. The levels of IL-10 and IL-12 in serum of CHI3L1 gene rs4950928 locus GC genotype and GG genotype in asthma group were lower than those in CC genotype. There was a significant difference between the two groups (P < 0.05). There was no significant difference in the serum TGF- 尾 _ 1 CHI3L1 rs4950928 locus GC genotypes between the two groups. There was no significant difference in the contents of IL-12, IL-12, IL-17 and IL-22 between the two groups compared with the GG genotypes. Conclusion the rs4950928 polymorphism of the 1: CHI3L1 gene may be the susceptibility factor of bronchial asthma in children. The genotype GCGG of rs4950928 may aggravate airway remodeling and inflammatory reaction in asthmatic children.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R725.6

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