不同氮素形态培养对枳橙幼苗硝酸还原酶活性及相关基因表达的影响

发布时间：2018-05-04 23:31

本文选题：枳橙 + 硝态氮　；参考：《果树学报》2017年04期

【摘要】：【目的】进一步探讨枳橙对氮素吸收利用机制,为柑橘生产上提高氮素利用效率和科学施肥提供科学依据。【方法】以枳橙为材料,采用水培方法,在Hoagland配方的基础上进行调节,研究了不同形态氮素配比对枳橙幼苗硝酸还原酶(NR)活性变化及相关基因表达量的影响。【结果】幼苗叶片及根系NR活性均随着培养时间的延长而增加,且叶片NR活性高于根系。其中2号(NO_3~-∶NH_4~+=7∶3)和3号(NO_3~-∶NH_4~+=5∶5)处理的叶片酶活最高;1号(NO_3~-∶NH_4~+=10∶0)和2号(NO_3~-∶NH_4~+=7∶3)处理根系的NR活性显著高于其他处理;进一步研究NR相关基因表达情况表明:3个基因(CitNia1、CitNia2、CitNia OA)在不同处理的枳橙幼苗体内均有表达,其中CitNia1和CitNia2分别是根系和叶片NR相关主效基因,2者均受到NO_3~-、NH_4~+配比诱导,且3号处理(NO_3~-∶NH_4~+=5∶5)下表达量最高。【结论】枳橙幼苗硝态氮代谢的主要部位为叶片,且CitNia2是主效基因,通过调节铵硝比例可以促进其表达。
[Abstract]:[objective] to further explore the mechanism of nitrogen absorption and utilization in citrange, so as to provide scientific basis for improving nitrogen use efficiency and scientific fertilization in citrus production. Based on the Hoagland formula, The effects of different forms of nitrogen on nitrate reductase (NR) activity and the expression of related genes were studied. [results] the NR activity of leaves and roots increased with the increase of culture time, and the NR activity of leaves was higher than that of roots. The highest enzyme activity was found in the leaves treated by the two kinds of treatments, No3 (1) and 10: 10 (10: 10: 10: 10: 0) and No3 (No3) and (3) No3 / S / NH4 ~ 5: 5), respectively. The NR activity of the roots of the two treatments was significantly higher than that of the other treatments in the treatment of No3 + 10: 10: 10: 0) and in the treatment of No.2 / No3 / S / VNH4 ~ 7w / 3), respectively, in which the activity of NR was significantly higher than that of the other treatments. Further study on the expression of NR related genes showed that CitNia1 CitNia2 CitNia OAA was expressed in different treatments of CitNia2, and CitNia1 and CitNia2 were induced by NO3 + -NH4- + ratio in roots and leaves, respectively. The expression of nitrate nitrogen in trifoliate orange seedlings was the highest under the condition of No3 +-10% NH _ 4 ~ 5: 5. [conclusion] the main part of nitrate nitrogen metabolism in trifoliate orange seedlings was leaf, and CitNia2 was a major gene, which could be promoted by adjusting the ratio of ammonium nitrate.
【作者单位】：湖南农业大学园艺园林学院;中南林业科技大学林学院;
【基金】：现代农业(柑橘)产业技术体系(CARS-027) 国家自然科学基金(31401824) 教育部高校博士点基金(20134320120006)
【分类号】：S666.4

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