PRPS1基因在儿童急性白血病中的表达及其临床意义

发布时间：2018-05-05 06:36

本文选题：PRPS基因 + 儿童急性白血病　；参考：《中国实验血液学杂志》2017年02期

【摘要】：目的:研究PRPS1基因在儿童急性白血病(AL)中的表达及其与该病临床特征的相关性。方法:采用实时荧光定量PCR和Western blot的方法分别检测176例儿童AL(初诊126例,完全缓解50例)中PRPS1 mRNA和蛋白的表达水平,分析其表达水平与临床特征的相关性。以21例非恶性血液病患儿骨髓作为对照。结果:①在BALL组中,PRPS1在初诊组表达水平显著高于对照组,同时高于完全缓解组(P均0.0001);在T-ALL及AML组中,仅在初诊组与完全缓解组中有差异(P均0.0001);②在B-ALL样本中,PRPS1表达水平随危险度升高而增加(P0.01);在T-ALL中各危险度分层组间则无明显差异(P0.05);在AML样本中,仅低危组与高危组差异具有统计学意义(P0.05);③PRPS1高表达与B-ALL患儿的高WBC计数及MRD阳性相关(P=0.020,P=0.026);4PRPS1与之前证实的复发相关基因NT5C2表达水平呈正相关(P0.05)。结论:PRPS1是儿童B-ALL预后不良相关危险因素,有望成为判断预后,指导个性化化疗的指标。
[Abstract]:Objective: to study the expression of PRPS1 gene in childhood acute leukemia and its correlation with clinical features. Methods: the expression levels of PRPS1 mRNA and protein in 176 children with ALL (126 cases at first diagnosis and 50 cases with complete remission) were detected by real-time fluorescence quantitative PCR and Western blot respectively, and the correlation between the expression level and clinical features was analyzed. Bone marrow of 21 children with non-malignant hematopathy was used as control. Results the expression of 1 / 1 in BALL group was significantly higher than that in the control group and in the complete remission group (P = 0.0001), and in T-ALL and AML group, the expression level was significantly higher than that in the control group. Only in the first diagnosis group and complete remission group there were differences (P = 0.0001). The expression level of B-ALL increased with the increase of risk, but there was no significant difference among the risk stratified groups in T-ALL, and in the AML sample, there was no significant difference between the two groups, and in the AML sample, there was no significant difference between the different risk stratified groups, and in the AML sample, there was no significant difference between the two groups. The high expression of PRPS1 in low risk group and high risk group was significantly higher than that in high risk group. There was a positive correlation between the high expression of PRPS1 and the high WBC count of children with B-ALL and the MRD positive correlation between P0. 020 and P0. 026. 4PRPS1 and the NT5C2 expression level of the previously confirmed recurrence related gene (P0. 05). Conclusion the ratio PRPS1 is a risk factor for poor prognosis of children with B-ALL, which is expected to be an index for judging prognosis and guiding individualized chemotherapy.
【作者单位】：重庆医科大学附属儿童医院血液肿瘤科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地儿科学重庆市重点实验室;
【基金】：重庆医科大学附属儿童医院转化医学研究项目(7000030)
【分类号】：R733.71

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