血瘀证与NF-κB信号通路相关基因表达相关性的PCR芯片技术分析

发布时间：2018-05-05 12:52

本文选题：PCR芯片 + 血瘀证　；参考：《时珍国医国药》2017年05期

【摘要】：目的 :探讨血瘀证与NF-κB信号通路相关基因表达的相关性。方法用高血压病血瘀证患者和非高血压病血瘀证患者血清作用于体外培养的人脐静脉血管内皮细胞,应用NF-κB信号通路PCR芯片技术,观察内皮细胞NF-κB信号通路相关基因表达的变化。结果在84个NF-κB信号通路相关基因中,高血压病血瘀证组与健康对照组比较,共检测到73个差异基因,其中,54个基因表达上调,19个基因表达下调。有6个上调基因和1个下调基因显示统计学差异(P0.05),上调基因包括EGR1、FOS、IKBKG、TLR9、TNFRSF10A、TRADD,下调基因为BCL2A1。非高血压病血瘀证组与健康对照组比较,共检测到71个差异基因,其中,49个基因表达上调,22个基因表达下调。有5个上调基因显示统计学差异(P0.05),包括IKBKG、NFKBIA、REL、TLR9、TRADD。可以推测:血瘀证的差异基因为IKBKG、TLR9、TRADD,均为上调基因。结论血瘀证与NF-B信号通路密切相关,其形成机制与炎症、免疫、凋亡等病理生理过程有关。
[Abstract]:Objective: to investigate the correlation between blood stasis syndrome and NF- 魏 B signaling pathway related gene expression. Methods the human umbilical vein endothelial cells cultured in vitro were treated with serum from patients with hypertensive blood stasis syndrome and patients with non-hypertension blood stasis syndrome. NF- 魏 B signal pathway PCR microarray technique was used. To observe the changes of NF- 魏 B signaling pathway related gene expression in endothelial cells. Results among the 84 NF- 魏 B signaling pathway related genes, 73 differentially expressed genes were detected in hypertensive blood stasis syndrome group and healthy control group. Among them, 54 genes were up-regulated and 19 genes were down-regulated. Six up-regulated genes and one down-regulated gene showed significant difference (P0.05). The up-regulated genes included EGR1, TLR9, TNFRSF10ATRADDD, and down-regulated gene BCL2A1. A total of 71 differentially expressed genes were detected in non-hypertensive blood stasis syndrome group and healthy control group. Among them, 49 genes were up-regulated and 22 genes were down-regulated. There were five up-regulated genes showing statistical difference (P0.05), including IKBKGN NFKBIAN TLR9 TRADD. It can be inferred that the differential basis of blood stasis syndrome is that IKBKGN TLR9 TRADDD are up-regulated genes. Conclusion Blood stasis syndrome is closely related to NF-B signaling pathway, and its mechanism is related to inflammation, immunity, apoptosis and other pathophysiological processes.
【作者单位】：广西中医药大学;广西中医药大学第二附属医院;
【基金】：广西壮族自治区中医药管理局中医药科技专项课题(No.GZKZ10-039) 广西中医药大学重点课题(No.ZD2010005)
【分类号】：R259

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