小偃6号TaWRKY45基因在高温抗条锈病中的功能研究

发布时间：2018-05-06 03:21

  本文选题：小偃号 + 高温　； 参考：《麦类作物学报》2017年10期

【摘要】：小偃6号是一个具有全生育期高温抗条锈性的小麦品种。在小麦抗病研究中发现TaWRKY45转录因子参与了对多种病害的调控。为了研究TaWRKY45在小偃6号高温抗条锈过程中的功能,以小偃6号为试验材料,通过实时荧光定量PCR技术(Real-time quantitative PCR,qRT-PCR)研究TaWRKY45的表达模式,并利用病毒诱导的基因沉默(VIGS)技术,研究了TaWRKY45沉默后对小偃6号高温抗条锈病的影响。结果表明,TaWRKY45在小麦根、茎、叶中均有表达,根中的表达量最高。当小麦受到水杨酸(SA)、条锈菌、高温(20℃)胁迫时,TaWRKY45表达量均上调。对小麦TaWRKY45基因进行沉默处理后,沉默叶片(BSMV:TaWRKY45-as)的条锈菌菌落增长,坏死细胞数目减少。TaWRKY45正向参与了小偃6号的高温抗条锈病过程。
[Abstract]:Xiaoyan 6 is a wheat variety with high temperature resistance to stripe rust in the whole growth period. In wheat disease resistance studies, TaWRKY45 transcription factors were found to be involved in the regulation of many diseases. In order to study the function of TaWRKY45 in the process of high temperature resistance to stripe rust of Xiaoyan 6, the expression pattern of TaWRKY45 was studied by real-time quantitative PCR technique (Real-time quantitative PCRQRT-PCRR), and the virus induced gene silencing (VIGS) technique was used. The effect of TaWRKY45 silencing on high temperature resistance to stripe rust of Xiaoyan 6 was studied. The results showed that TaWRKY45 was expressed in the roots, stems and leaves of wheat, and the highest expression was found in the roots. The expression of TaWRKY45 was upregulated when wheat was stressed by salicylate, stripe rust and high temperature (20 鈩，

本文编号：1850559