罗氏沼虾TLRs基因表达差异分析

发布时间：2018-05-06 03:28

本文选题：罗氏沼虾 + Toll样受体(TLRs)　；参考：《南方农业学报》2017年04期

【摘要】：【目的】探究罗氏沼虾Toll样受体(TLRs)基因(MrToll1、MrToll2和MrToll3)在不同组织中及感染溶藻弧菌(Vibrio alginolyticus)后不同时间鳃组织的表达量差异变化,为揭示罗氏沼虾TLRs在应对病害等免疫方面的表现和作用机理提供参考依据。【方法】利用实时荧光定量RT-PCR分别检测MrToll1、MrToll2和MrToll3基因在罗氏沼虾肌肉、鳃、肝胰腺、心脏、胃、肠、血淋巴、神经节、眼柄等不同组织中的相对表达量,并以同样方法检测罗氏沼虾感染溶藻弧菌后TLRs基因的相对表达量变化情况。【结果】罗氏沼虾MrToll1、MrToll2和MrToll3基因在各组织中广泛表达,但存在组织表达差异性。MrToll1和MrToll2基因在鳃组织中的相对表达量最高,分别是在肝胰腺中相对表达量的19.26和20.03倍;MrToll3基因在神经节中的相对表达量最高,是在肝胰腺中的10.13倍。感染溶藻弧菌悬液后,罗氏沼虾死亡率随时间的推移不断升高,至注射感染72 h时死亡率达峰值(64%),随后不再出现死亡。感染溶藻弧菌后罗氏沼虾MrToll1基因表达量呈先升高后下降的变化趋势,注射感染后24 h的表达量达峰值;Mrtoll2基因表达量呈下降—升高—下降—升高的波动变化趋势,注射感染后24 h的表达量达峰值;MrToll3基因表达量急剧下降,且维持在较低水平。【结论】罗氏沼虾MrToll1基因是鳃组织抵御细菌感染的主要应答基因,MrToll2基因可能参与鳃组织的其他免疫活动,MrToll3基因的调控机制尚未清晰。
[Abstract]:[objective] to investigate the changes of expression of Toll like receptor (TLRs) gene of Macrobrachium rosenbergii, Mr Toll1, Mr Toll2 and Mr Toll3, in different tissues and in Gill tissues after infection with Vibrio alginolyticus. [methods] Real-time fluorescence quantitative RT-PCR was used to detect the expression of MRToll1, MRToll2 and MrToll3 gene in muscle, Gill, liver, pancreas, heart, stomach and intestine of Macrobrachium rosenbergii. Relative expression in different tissues such as hemolymph, ganglion, eye handle, etc. The relative expression of TLRs gene was detected by the same method after infection with Vibrio alginolyticus. [results] the genes of MRToll1, MRToll2 and MrToll3 were widely expressed in various tissues. The relative expression of MRToll1 and MrToll2 gene in Gill tissue was 19.26 and 20.03 times higher than that in hepatopancreas, respectively. The relative expression of MRToll3 gene in ganglion and hepatopancreas was higher than that in hepatopancreas and was 10.13 times higher than that in hepatopancreas. After infection with Vibrio alginolyticus suspension, the mortality rate of Macrobrachium rosenbergii increased with the passage of time, and reached the peak at 72 h after injection, and then no death occurred. The MrToll1 gene expression of Macrobrachium rosenbergii increased first and then decreased after infection with Vibrio alginolyticus. The expression of MRtoll2 gene reached the peak at 24 h after injection. The expression of Mr Toll3 gene decreased sharply at 24 h after injection. [conclusion] the MrToll1 gene of Macrobrachium rosenbergii is the main response gene to resist bacterial infection in the Gill tissue. The regulation mechanism of MRToll3 gene may be involved in other immune activities in the Gill tissue of Macrobrachium rosenbergii.
【作者单位】：上海海洋大学水产与生命学院;上海申漕特种水产开发公司;
【基金】：上海市科学技术委员会重点攻关项目(11391901400) 上海市科技兴农重点攻关项目(沪农科攻字[2010]第1-6号) 上海市教育委员会重点学科建设项目(J50701)
【分类号】：S945.4

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