基因芯片技术分析转SlNAC10基因拟南芥非生物胁迫相关差异表达基因

发布时间：2018-05-06 06:35

本文选题：SlNAC + 基因芯片　；参考：《中国细胞生物学学报》2016年08期

【摘要】：NAC(NAM、ATAF1/2和CUC2)转录因子是植物特有的转录调控因子,在植物的器官建成、生长发育以及抵御非生物胁迫等方面发挥着至关重要的作用。该文利用基因芯片技术筛选转Sl NAC10基因拟南芥和野生型拟南芥非生物胁迫抗性相关差异表达基因,并通过实时荧光定量PCR对部分差异表达基因进行验证。芯片结果显示,差异表达2倍以上的基因有4 054个,其中与非生物胁迫相关基因有15个,与非生物胁迫相关的转录因子基因有14个,这些基因参与应答渗透胁迫、响应高盐、冷、热、高光强等胁迫。对差异表达2倍以上的基因进行GO(Gene Ontology)分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析,发现这些基因在非生物胁迫相关的13个注释中富集,涉及相关代谢途径96个,其中包括植物激素信号转导、精氨酸和脯氨酸代谢、吲哚生物碱合成、谷胱甘肽代谢等。以上结果表明,SlNAC10可直接或间接调控多种下游基因的表达,提高植物抵御非生物胁迫的能力。
[Abstract]:NAC NAMN ATAF 1 / 2 and CUC 2) transcription factors are plant specific transcription regulators, which play an important role in organ building, growth and development of plants and resistance to abiotic stress. The differentially expressed genes related to abiotic stress resistance of transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana were screened by gene chip technique, and some differentially expressed genes were verified by real-time fluorescence quantitative PCR. The microarray results showed that there were 4 054 differentially expressed genes, including 15 genes related to abiotic stress and 14 transcription factor genes related to abiotic stress. These genes were involved in the response to osmotic stress and responded to high salt. Cold, hot, high intensity and other stresses. GO(Gene Ontology) and KEGG(Kyoto Encyclopedia of Genes and Genomes) analysis showed that these genes were enriched in 13 notes related to abiotic stress and involved 96 related metabolic pathways, including plant hormone signal transduction. Arginine and proline metabolism, indole alkaloid synthesis, glutathione metabolism, etc. These results suggest that SlNAC10 can directly or indirectly regulate the expression of many downstream genes and improve the ability of plants to resist abiotic stress.
【作者单位】：辽宁师范大学生命科学学院辽宁省植物生物技术重点实验室;
【基金】：国家自然科学基金(批准号:31340052)资助的课题~~
【分类号】：Q943.2

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