大白菜表皮毛控制基因Brtri1的精细定位与候选基因分析

发布时间：2018-05-06 09:49

本文选题：大白菜 + 表皮毛　；参考：《沈阳农业大学》2016年硕士论文

【摘要】：大白菜(Brassica campestris L.ssp. pekinensis,染色体组AA,2n=2x=20)是我国种植面积最大、产量最高的蔬菜作物。表皮毛是植物表皮的衍生结构,是研究植物细胞分化的理想系统。本文通过利用无表皮毛大白菜DH系‘FT’和有表皮毛DH系'PurDH-1'构建分离群体,克隆了1个控制大白菜表皮毛的候选基因。1.利用‘FT’和'PurDH-1'杂交,构建了包含5027个单株的F2分离群体,其中3801个单株有表皮毛,1226个单株无表皮毛,证实大白菜有表皮毛性状受单显性基因控制,命名为Brtril。2.利用F2分离群体中的1226株无表皮毛植株作为定位群体,最终将Brtril定位在A06染色体上分子标记pur6-31与pur6-39之间,物理距离为16.84 kb。基因预测表明,该该区域共有4个完整的ORF。其中,Bra025311编码MYB转录因子,与拟南芥表皮毛调控基因GLl高度同源。因此,将Bra025311作为Brtril的候选基因。3.序列分析表明,‘FT’中Bra025311基因的第3外显子发生5bp的缺失事件,造成移码突变。4.表达模式分析表明,Bra025311在'PurDH-1'正常表达,但是在‘FT’上不表达。5.利用第三外显子的序列差异,开发出1个共显性Indel标记,能够有效区分杂合基因型和纯合基因型,可用于无表皮毛性状的分子标记辅助选择。
[Abstract]:Chinese cabbage (Brassica campestris L.ssp. pekinensis) is the largest vegetable crop in China. Epidermis is a derived structure of plant epidermis and an ideal system for studying plant cell differentiation. In this paper, a candidate gene for controlling epidermal hair of Chinese cabbage (Brassica campestris L.) was cloned by using the DH line FT' and the epidermal DH line PurDH-1'of Brassica campestris L. A F2 segregation population of 5027 plants was constructed by using FT' and PurDH-1' hybrids, of which 3801 plants had epidermal hairs and 1226 plants had no epidermal hairs. It was confirmed that the epidermal traits of Chinese cabbage were controlled by a single dominant gene, named Brtril.2. Using 1226 epidermal fur plants from F _ 2 segregated population as the mapping population, the Brtril was mapped between the molecular marker pur6-31 and pur6-39 on chromosome A06, and the physical distance was 16.84 kb. Gene prediction showed that there were four complete ORFs in this region. Bra025311 encodes MYB transcription factor, which is highly homologous to Arabidopsis epidermal regulatory gene GLl. Therefore, Bra025311 is used as the candidate gene of Brtril. Sequence analysis showed that 5bp deletion occurred in exon 3 of Bra025311 gene, resulting in frameshift mutation. 4. The expression pattern analysis showed that Bra025311 was expressed normally in PurDH-1', but not in FT'. Using the sequence difference of exon 3, a codominant Indel marker was developed, which can effectively distinguish heterozygous genotype from homozygous genotype, and can be used for molecular marker-assisted selection of fur traits without surface.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S634.1

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