新疆汉族及维吾尔族结直肠癌原发灶与转移灶中KRAS基因突变分析

发布时间：2018-05-06 16:09

  本文选题：结直肠肿瘤 + KRAS基因　； 参考：《临床与实验病理学杂志》2017年02期

【摘要】：目的观察新疆地区汉族、维吾尔族人群中结直肠癌(colorectal cancer,CRC)原发灶与转移灶中KRAS基因状态的差异及其与CRC患者临床病理特征之间的关系。方法收集2012年~2014年间新疆地区汉族及维吾尔族CRC标本189例,其中52例有配对转移灶。采用突变扩增阻滞系统(amplification refractory mutation system,ARMS)进行KRAS基因12、13密码子已知常见突变位点检测。结果KRAS基因在CRC原发灶病例中汉族、维吾尔族的突变率分别为42.8%(62/145)、43.2%(19/44),两者差异无显著性(P0.05),T3/T4期及有淋巴结转移的病例中突变率较高(P0.05),但与患者性别、民族、年龄、分化程度、TNM分期、有无远处转移、原发灶所在的部位均无相关性(P0.05)。KRAS基因在CRC转移灶中汉族、维吾尔族的突变率分别为33.3%(12/36)、31.3%(5/16),两者差异无显著性(P0.05),并发现年龄≥65岁者突变率较高(P0.05),与所研究的其他临床病理特征无相关性。52例转移灶中KRAS基因的突变率为32.7%(17/52),而对应的原发灶突变率达50%(26/52),明显高于转移灶(P0.000 1),转移灶突变类型与原发灶一致。结论 KRAS基因在浸润较深、有淋巴结转移及65岁以上发生转移的CRC中突变率较高,在原发灶的突变率高于配对转移灶。KRAS基因突变率在新疆汉族、维吾尔族CRC患者中差异无显著性,提示KRAS基因突变促进CRC的进展,并且在原发灶中更易发生突变,但汉族、维吾尔族间无差异。
[Abstract]:Objective to observe the difference of KRAS gene status between primary and metastatic colorectal cancer in Han and Uygur populations in Xinjiang and its relationship with clinicopathological features of CRC patients. Methods 189 CRC specimens from Han and Uygur nationalities in Xinjiang were collected from 2012 to 2014, of which 52 cases had pairing metastatic foci. The mutation amplification block system amplification refractory mutation system ARMS was used to detect the known common mutation sites of the KRAS gene codon 12n13. Results the mutation rates of KRAS gene in Han and Uygur patients with primary CRC were 42. 8 and 42 / 145, respectively. There was no significant difference between the two groups. The mutation rate of KRAS gene in patients with lymph node metastasis was higher than that in patients with lymph node metastasis (P 0. 05), but it was associated with sex, nationality, age, differentiation and TNM stage. There was no correlation between the location of the primary tumor and the distant metastasis. KRAS gene was found in the Han nationality in the CRC metastasis. The mutation rate of Uygur nationality was 33.32 / 36 / 31.30.There was no significant difference between the two groups (P 0.05), and it was found that the mutation rate of KRAS gene was higher in the age 鈮，

本文编号：1852977