吡格列酮提高C57bl小鼠胆盐肠肝循环相关基因的表达

发布时间：2018-05-08 14:09

本文选题：吡格列酮 + 胆盐　；参考：《南京医科大学学报(自然科学版)》2017年12期

【摘要】：目的 :观察吡格列酮对小鼠胆盐肠肝循环转运相关基因的表达影响。方法 :将16只小鼠随机分为两组:对照组(normal control,NC)、药物组(medicine,M),分别给予普通饮食、普通饮食加吡格列酮(pioglitazone)灌胃,喂养10周后处死小鼠取血、胆汁、小鼠肝脏、小肠组织,生化分析仪分析小鼠血样代谢指标,q RT-PCR技术检测各组小鼠组织内胆盐肠肝循环相关基因的表达。结果:M组小鼠胆汁中胆盐含量NC组显著升高,肝脏法尼醇X受体(farnesoid X receptor,FXR)及胆盐合成相关基因胆固醇7羟化酶(cytochrome P450 enzyme7 A1,CYP7A1)、胆固醇27羟化酶(CYP7A1cytochrome P450 enzyme27 A1,CYP27A1)以及胆盐转运基因胆盐输出泵基因(bile salt export pump,BSEP)、牛黄胆酸钠转运基因(sodium/taurocholate cotransporting polypeptide,NTCP)的表达较NC组也明显升高(P0.01)。肝脏组织免疫组化证实了M组BSEP在蛋白层面表达升高。M组小肠组织内胆盐转运基因顶膜钠离子依赖性胆汁酸转运体(apical sodium dependent bile acid transporter,ASBT)的表达较NC组明显升高(P0.01)。结论 :吡格列酮能够诱导胆盐合成相关基因的表达,并上调胆盐在肝脏以及小肠内的转运相关基因的表达,在转录水平参与胆盐肠肝循环的调控。
[Abstract]:Aim: to observe the effect of pioglitazone on the expression of bile salt enterohepatic circulatory transport related genes in mice. Methods: sixteen mice were randomly divided into two groups: control group (normal control group), control group (control group), medicine group (medicine), which were given common diet, common diet and pioglitazone respectively. After 10 weeks of feeding, the mice were killed to take blood, bile, liver and small intestine tissue. Biochemical analyzer was used to detect the expression of bile salt enterohepatic circulation related genes in the tissues of mice by Q RT-PCR technique. Results the content of bile salt in group C was significantly higher than that in group C. Liver farnesoid X receptor (FXR) and cholesterol 7 hydroxylase associated with bile salt biosynthesis, cytochrome P450 enzyme7 A1, CYP7A1, CYP7A1 cytochrome P450 enzyme27 A1, CYP27A1) and bile salt export pumpBSEP1, sodium oxalate transport gene The expression of Taurocholate cotransporting polypeptidec was significantly higher than that of NC group. Liver tissue immunohistochemistry confirmed that the expression of BSEP at protein level in group M was higher than that in group C. The expression of apical sodium dependent bile acid transporter was significantly higher in group M than that in group C (P 0.01), and the expression of sodium ion-dependent bile acid transporter gene in the intestinal tissue of group M was significantly higher than that in group M. Conclusion: pioglitazone can induce the expression of bile salt biosynthesis related genes and up-regulate the expression of bile salt transportation-related genes in the liver and small intestine and participate in the regulation of bile salt enterohepatic circulation at the transcriptional level.
【作者单位】：南京医科大学第一附属医院结直肠外科;
【基金】：国家自然科学基金面上项目(81470881)
【分类号】：R965

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