杨树MYB转录因子家族基因应答盐胁迫表达特性分析

发布时间：2018-05-11 03:10

  本文选题：杨树 + MYB转录因子　； 参考：《植物研究》2017年03期

【摘要】：MYB转录因子家族是植物中数量最多的转录因子家族之一,在植物次生代谢调节、信号转导和抗逆等生物过程起重要作用。根据MYB转录因子结构域组成差异可分4个亚家族:即1R-MYB(MYB-relaed)、R2R3-MYB、3R-MYB和4R-MYB。其中,R2R3-MYB亚家族数量最多,可进一步分为22个亚组;利用生物信息学分析杨树MYB转录因子蛋白序列的保守结构域、系统发生、基因组定位、氨基酸组成和理化性质等;参照拟南芥MYB转录因子功能,预测杨树MYB转录因子功能;基于84K杨转录组测序和RT-qPCR分析,从301个杨树MYB转录因子基因中筛选出69个应答盐胁迫基因(P≤0.05)。其中,上调表达基因32个,下调表达基因37个。该研究可为进一步研究杨树MYB家族基因功能提供参考依据。
[Abstract]:MYB transcription factor family is one of the most abundant transcription factors in plants, which plays an important role in plant secondary metabolism regulation, signal transduction and stress resistance. According to the difference of MYB transcription factor domain composition, there are four subfamilies: R2R3-MYBC3R-MYB and 4R-MYB. The number of R2R3-MYB subfamily was the most, which could be further divided into 22 subgroups. The conserved domain, phylogeny, genome location, amino acid composition and physicochemical properties of MYB transcription factor protein sequence in poplar were analyzed by bioinformatics. According to the MYB transcription factor function of Arabidopsis thaliana, the MYB transcription factor function of poplar was predicted. Based on the transcription sequence and RT-qPCR analysis of 84K poplar, 69 genes responding to salt stress were screened out of 301 MYB transcription factor genes (P 鈮，

本文编号：1872142