Sox2和Cdx2基因编码蛋白在胃癌及其癌前病变中的表达及意义

发布时间：2018-05-11 07:01

本文选题：Sox + Cdx　；参考：《现代肿瘤医学》2016年10期

【摘要】：目的:采用免疫组化法观察研究Sox2和Cdx2基因编码蛋白在胃癌及其癌前病变组织中的表达并分析其与胃癌临床病理生物学行为的关系,探讨Sox2、Cdx2异常表达在胃癌发生发展中的作用机制及相互关系。方法:收集中国医科大学附属第一医院肿瘤外科手术切除胃癌标本257例,采用Envision免疫组织化学染色方法和HID-ABp H2.5-PAS黏蛋白组织化学染色法,观察Sox2和Cdx2在胃癌及其癌前病变中的表达,分析其表达的相互关系。结果:胃癌组织中Sox2的阳性表达率(64.98%)显著低于正常胃黏膜(89.02%)、肠上皮化生(76.38%)和异型增生(71.01%)中的阳性表达率,P均0.05;弥漫型胃癌(71.54%)中的表达显著高于肠型胃癌( 58. 25% ) ,P 0. 05。Cdx2 蛋白在胃癌组织中的阳性表达率( 53. 93% ) 明显高于正常胃黏膜中的阳性表达率( 6. 74% ) ,在肠上皮化生组织中的阳性表达率( 88. 89% ) 显著高于胃癌组织的阳性表达率,肠型胃癌中( 68. 75% ) 的表达水平高于弥漫型胃癌( 40. 38% ) ,P 均 0. 05。结论: Sox2 对胃表型的维持起重要作用,可能与弥漫型胃癌的发生关系更为密切。Cdx2 对肠表型的维持起重要作用,可能与肠型胃癌的发生更为密切。中的表达显著高于肠型胃癌(58.25%),P0.05。Cdx2蛋白在胃癌组织中的阳性表达率(53.93%)明显高于正常胃黏膜中的阳性表达率(6.74%),在肠上皮化生组织中的阳性表达率(88.89%)显著高于胃癌组织的阳性表达率,肠型胃癌中(68.75%)的表达水平高于弥漫型胃癌(40.38%),P均0.05。结论:Sox2对胃表型的维持起重要作用,可能与弥漫型胃癌的发生关系更为密切。Cdx2对肠表型的维持起重要作用,可能与肠型胃癌的发生更为密切。
[Abstract]:Objective: to study the expression of Sox2 and Cdx2 gene coding proteins in gastric carcinoma and its precancerous lesions by immunohistochemical method, and to analyze the relationship between the expression and clinicopathologic behavior of gastric cancer. To investigate the mechanism and relationship of abnormal expression of Sox2 and Cdx2 in gastric carcinogenesis. Methods: a total of 257 gastric cancer specimens were collected from the first affiliated Hospital of China Medical University. Envision immunohistochemical staining and HID-ABp H2.5-PAS mucin histochemical staining were used. To observe the expression of Sox2 and Cdx2 in gastric carcinoma and its precancerous lesions and to analyze the relationship between them. Results: the positive expression rate of Sox2 in gastric carcinoma (64.98) was significantly lower than that in normal gastric mucosa (89.02), intestinal metaplasia (76.38) and dysplasia (71.01) (P 0.05), and the expression of Sox2 in diffuse gastric carcinoma (71.54) was significantly higher than that in intestinal type (58). 25%). The positive expression rate of 05.Cdx2 protein in gastric cancer tissues (53. 5%). The positive expression rate in normal gastric mucosa was significantly higher than that in normal gastric mucosa (93%) (6. 5%). The positive expression rate in intestinal metaplasia tissues (88. 8%). The positive rate of gastric cancer was significantly higher than that of gastric carcinoma (89%), and the positive rate of intestinal type gastric cancer (68.8%). The expression level in 75% of cases was higher than that in diffuse gastric carcinoma (40. 5%). 38%). 05. Conclusion: Sox2 may play an important role in the maintenance of gastric phenotype, and may play an important role in the maintenance of intestinal phenotype and may be more closely related to the occurrence of diffuse gastric cancer. The positive expression rate of P0.05.Cdx2 protein in gastric carcinoma was significantly higher than that in normal gastric mucosa (53.93) and that in intestinal metaplasia (88.89) was significantly higher than that in gastric carcinoma. In intestinal type gastric carcinoma, the expression level was higher than that in diffuse type gastric carcinoma (40.38%, P < 0.05). Conclusion the weight Sox2 may play an important role in the maintenance of gastric phenotype, and CDX2 may play an important role in the maintenance of intestinal phenotype and may be more closely related to the occurrence of diffuse gastric carcinoma.
【作者单位】：中国医科大学附属第一医院肿瘤研究所胃肠肿瘤病理研究室普通外科研究所肿瘤病理研究室;
【基金】：国家自然科学基金(编号:81071650,30973503) 辽宁省科技厅科学技术计划项目(编号:2013225303-103,2011404013-3) 辽宁省高等学校攀登学者计划(编号:2009-2012)
【分类号】：R735.2

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