大豆5个新发现ERF基因的生物信息学及表达分析

发布时间：2018-05-12 10:39

本文选题：大豆 + ERF转录因子　；参考：《浙江农业学报》2016年10期

【摘要】：ERF转录因子广泛存在于植物中并且参与植物对生物及非生物胁迫的响应。从NCBI数据库中获得5个新的ERF基因,Gm ERFa/b/c/d/e。蛋白序列分析显示,5个ERF基因均含有一个保守的AP2/ERF结合域。进化分析表明,Gm ERFe与Gm ERF3和Gm ERF7同源性最高,Gm ERFb、Gm ERFc与Gm ERF5的同源性最高,Gm ERFd与Gm EREBP1的同源性较高,而Gm ERFa与其他ERF蛋白的同源性均较低。实时荧光定量PCR结果显示,5个基因都主要在大豆的根和叶中表达。逆境处理后的实时荧光定量PCR结果显示,Gm ERFd/e主要对乙烯信号和低温产生响应,Gm ERFb主要对干旱产生响应,而Gm ERFa主要对低温产生响应。
[Abstract]:ERF transcription factors exist widely in plants and participate in plant response to biological and abiotic stress. Five new ERF genes were obtained from the NCBI database. Protein sequence analysis showed that all five ERF genes contained a conserved AP2/ERF binding domain. Evolutionary analysis showed that GM ERFe had the highest homology with GM ERF3 and GM ERF7. The homology between GM ERFd and GM ERF5 was higher than that between GM ERFd and GM EREBP1, but the homology between GM ERFa and other ERF proteins was lower. The results of real-time fluorescence quantitative PCR showed that the five genes were mainly expressed in the roots and leaves of soybean. The results of real-time fluorescence quantitative PCR showed that GM ERFd/e was mainly responsive to ethylene signal and low temperature. GM ERFb was mainly responsive to drought, while GM ERFa was mainly responsive to low temperature.
【作者单位】：齐齐哈尔大学生命科学与农林学院;黑龙江省兽医科学研究所;
【基金】：国家自然科学基金项目(31301335) 黑龙江省教育厅科学技术研究项目(12541889) 黑龙江省自然科学基金项目(C201458)
【分类号】：Q943.2

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