芽孢杆菌基本特征、16S rRNA对比分析及特异性基因挖掘

发布时间：2018-05-13 01:24

本文选题：芽孢杆菌 + S　；参考：《基因组学与应用生物学》2017年11期

【摘要】：为了研究10种芽孢杆菌的基本特性,下载到70条芽孢杆菌属细菌的16S r RNA序列,通过对比芽孢杆菌的基本特征和16S r RNA序列种内和种间相似性,发现基本特征很难区分这10种菌株。16S r RNA序列种内相似性最高为100%,最低的是B.amyloliquefaciens,种内分化较明显,最低值为90.85%,种间相似性最高的2个菌株是B.subtilis strain 168和B.amyloliquefaciens strain BCRC 11601,最高值为99.66%,最低为92.01%。B.subtilis、B.amyloliquefaciens、B.anthracis、B.cereus、B.methylotrophicus、B.atrophaeus之间某些菌株的16S r RNA相似性极高,通过16S r RNA序列很难区分,B.coagulans的16S r RNA特异性最好。同时本研究提供了gyr A、gyr B、rpo A、yya R、yya O等多个能够准确鉴定的特异性基因,为快速鉴定菌种提供了便利。
[Abstract]:In order to study the basic characteristics of 10 kinds of Bacillus, the 16s r RNA sequences of 70 strains of Bacillus were downloaded. The basic characteristics of Bacillus and the similarity between species and species of 16s r RNA sequences were compared. It was found that it was difficult to distinguish the basic characteristics of the 10 strains. The similarity of the 10 strains was 100%, and the lowest was B. amylolique faciens. The lowest value was 90.85, the two strains with the highest similarity among species were B.subtilis strain 168 and B.amyloliquefaciens strain BCRC 11601, the highest value was 99.66, and the lowest value was 92.01. B. subtilisa B.amyloliquefaciens B.cereusus B. methylotrophicus B.atrophaeus had the highest similarity of 16s r RNA. It was difficult to distinguish the specificity of 16s r RNA between B. coagulans by 16s r RNA sequence. At the same time, this study provided several specific genes, such as gyr Aurgyr Bnrpo Amoyya Rnyya O and so on, which could be accurately identified, and thus provided convenience for rapid identification of bacteria species.
【作者单位】：海南省农业科学院植物保护研究所海南省植物病虫害防控重点实验室;
【基金】：海南省重点研发计划(ZDYF2016060) 省属科研院所技术开发研究专项(KYYS-2015-05)共同资助
【分类号】：Q933

【相似文献】