大肠癌围手术期患者阴虚证差异表达基因及信号通路分析

发布时间：2018-05-13 06:02

本文选题：大肠癌围手术期 + 阴虚证　；参考：《中华中医药学刊》2017年06期

【摘要】：方法:针对大肠癌围手术期患者的主要中医证候阴虚证,采集符合条件病例的大肠癌组织及癌周正常组织,采用罗氏公司含12×135K个基因的人全基因组芯片检测大肠癌组织差异表达基因,筛选并分析阴虚证癌组织组和癌周正常组织组的差异表达基因,利用Pathway对相关基因进行生物信息学分析。结果:得出大肠癌围手术期患者阴虚证癌组织组和癌周正常组织组表达的差异基因共4869个,包括上调基因1574个,下调基因3295个。经检索数据库Gene Bank,获取基因名称等相关信息。进行Pathway分析,筛选出阴虚证癌组织组和癌周正常组织组之间具有显著差异表达基因主要涉及的信号转导通路。结论:大肠癌围手术期阴虚证癌组织组与癌周正常组织组比较,存在组织差异基因表达特征图谱,主要涉及主要涉及炎症反应,细胞内吞功能,矿物质吸收,酮体合成与分解功能,免疫功能等相关基因。其主要信号转导通路包括MAPK信号通路及p53信号通路等。
[Abstract]:Methods: according to the main syndromes of yin deficiency in patients with colorectal cancer during perioperative period, the tissues of colorectal cancer and normal tissues around cancer were collected. The differentially expressed genes in colorectal cancer tissues were detected by human genome microarray with 12 脳 135K genes in Roche Company. The differentially expressed genes were screened and analyzed in the cancer tissues of Yin-deficiency syndrome and the normal tissues around cancer. The related genes were analyzed by bioinformatics using Pathway. Results: there were 4869 differentially expressed genes including 1574 up-regulated genes and 3295 down-regulated genes. The database Gene Bank was searched for gene name and other related information. By Pathway analysis, the signal transduction pathways involved in the significant difference of expression genes between cancer tissues of yin deficiency syndrome and normal tissues around carcinoma were screened. Conclusion: there is a characteristic map of differentially expressed genes in the tissues of cancer with deficiency of yin and normal tissues around carcinoma of large intestine in perioperative period, which mainly involves inflammation, endocytosis and mineral absorption. Ketone synthesis and decomposition function, immune function and other related genes. Its main signal transduction pathways include MAPK signaling pathway and p53 signaling pathway.
【作者单位】：浙江省人民医院肿瘤内科;浙江中医药大学第一临床医学院;
【基金】：浙江省中医药优秀青年人才基金项目(2015ZQ003) 浙江省医学会临床科研基金项目(2015ZYC-B1)
【分类号】：R735.34

【相似文献】