疆岳驴催乳素受体基因多态性及其与泌乳性状间的相关性分析

发布时间：2018-05-13 15:21

本文选题：疆岳驴 + 催乳素受体基因　；参考：《中国畜牧兽医》2017年01期

【摘要】：试验旨在研究疆岳驴催乳素受体(prolactin receptor,PRLR)基因多态性及其与泌乳性状间的关系,探寻可用于疆岳驴选育的分子遗传标记。随机选取120头疆岳驴,对疆岳驴的泌乳性能指标进行测定,并运用PCR-SSCP方法对疆岳驴PRLR基因侧翼区进行多态性分析。结果显示,PRLR基因在疆岳驴群体中存在2种基因型:AA和AB,A为优势等位基因;测序结果显示,PRLR基因的碱基突变位置为g.29764584CG,该突变为同义突变,没有导致编码的氨基酸发生改变;经关联分析,PRLR基因AB基因型个体平均日泌乳量显著高于AA基因型(P0.05),AA基因型个体乳蛋白率显著高于AB基因型(P0.05),在乳糖率上差异不显著(P0.05)。疆岳驴PRLR基因突变与泌乳性状存在关联性,推测PRLR基因可以作为疆岳驴乳用型选育的分子遗传标记之一。
[Abstract]:The aim of the experiment was to study the polymorphism of prolactin receptor (PRLRR) gene and its relationship with lactation traits in Xinjiang Yue donkey, and to explore the molecular genetic markers that can be used in breeding of Xinjiang Yue donkey. A total of 120 donkeys from Xinjiang were randomly selected to determine the lactating performance and the polymorphism of PRLR gene flanking region was analyzed by PCR-SSCP method. The results showed that there were two dominant alleles of the PRLR gene in the Xinjiang Yue donkey population, and the sequence analysis showed that the base mutation position of the PRLR gene was g.29764584CG. the mutation was synonymous and did not result in the change of the encoded amino acid. By association analysis, the average daily milk yield of individuals with AB genotype of PRLR gene was significantly higher than that of individuals with AA genotype P0.05A genotype, and the rate of milk protein was significantly higher than that of genotype AB genotype P0.05A, but there was no significant difference in lactose rate between them. The mutation of PRLR gene was associated with lactation traits in Xinjiang Yue Donkey. It was suggested that PRLR gene could be used as one of the molecular genetic markers for breeding milk type of Xinjiang Yue Donkey.
【作者单位】：新疆农业大学动物科学学院;新疆畜牧科学院畜牧研究所;
【基金】：自治区高技术研究发展项目(201411106) 人社部留学人员科技活动资助启动类项目《驴泌乳相关功能基因表达调控机制研究》(新人社函〔2016〕438) 自治区科技兴新项目(2016023A06)
【分类号】：S822

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