携目的基因的靶向超声微泡对肝癌HepG2细胞增殖活性的影响

发布时间：2018-05-13 16:16

本文选题：肝肿瘤 + 细胞增殖　；参考：《重庆医学》2017年05期

【摘要】：目的制备一种靶向肝癌HepG2细胞的载单纯疱疹病毒胸腺嘧啶核苷激酶(HSV-TK)基因超声微泡,并考察其体外寻靶能力及对HepG2细胞的增殖抑制作用。方法用机械振荡法制备超声微泡,生物素-亲和素桥连方式构建靶向载HSVTK超声微泡。检测其一般特性,并进行体外实验检测其对HepG2细胞增殖的影响。结果靶向载HSV-TK超声微泡可较多地聚集在HepG2细胞表面,通过检测增殖细胞核抗原(PCNA)及噻唑蓝(MTT)法,发现载基因靶向微泡组的增殖能力明显下降,细胞凋亡明显增加,细胞侵袭实验表明载基因靶向微泡组(22.18±2.01)较对照组及载基因非靶向微泡组明显减少,对HepG2细胞增殖及侵袭能力有较好的抑制作用。结论携目的基因靶向超声微泡对肝癌HepG2细胞在体外有较好抑制效果。
[Abstract]:Objective to prepare an ultrasound microbubble carrying herpes simplex virus thymidine kinase (HSV-TK) gene containing herpes simplex virus (HSV) gene in HepG2 cells, and to investigate its target ability in vitro and to inhibit the proliferation of HepG2 cells. Methods the ultrasonic microbubbles were prepared by mechanical oscillatory method, and the target HSVTK ultrasound microbubbles were constructed by biotin avidin bridge. The effects on the proliferation of HepG2 cells were detected in vitro. Results the target loaded HSV-TK ultrasound microbubbles could accumulate more on the surface of HepG2 cells. By detecting proliferating cell nuclear antigen (PCNA) and thiazolium (MTT) method, it was found that the proliferation ability of the gene targeted microbubble group decreased obviously and the cell apoptosis increased obviously. The invasion experiment showed that the gene targeting microbubble group (22.18 + 2.01) decreased significantly compared with the control group and the non targeting microbubble group, and had a better inhibitory effect on the proliferation and invasion ability of HepG2 cells. Conclusion the target gene targeted ultrasound microbubbles have a good inhibitory effect on the HepG2 cells of liver cancer in vitro.

【作者单位】：重庆市渝北区人民医院外科;重庆医科大学附属第二医院普通外科;重庆市巴南区中医院;
【分类号】：R735.7

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