红肉桃两类花色素苷积累模式与相关基因表达差异

发布时间：2018-05-14 00:45

本文选题：红肉桃 + 花色素苷　；参考：《中国农业科学》2017年13期

【摘要】：【目的】分析中国主要红肉桃种质呈色类型及分子机理,为红肉桃种质优异基因发掘和分子标记辅助选择育种奠定理论基础。【方法】选择8份红肉桃种质和1份白肉桃种质为试材,分别在花后一个月开始采集样品,以后每隔10 d左右采集一次,至果实成熟期为止;用2%甲酸甲醇提取不同种质果肉的花色素苷,分别测定其在510 nm和700 nm的吸光值;利用实时荧光定量PCR(q RT-PCR)方法,测定与花色素苷合成相关的13个关键结构基因和调节基因的表达水平。【结果】根据果实发育中后期花色素苷含量的变化趋势可将8份红肉桃种质分为2类,一类种质的花色素苷合成高峰出现在果实成熟期(成熟期积累型),如‘郑引82-9’‘大红袍’‘黑布袋’‘红桃’和‘天津水蜜’;另一类种质的花色素苷合成高峰出现在果实发育中期,在成熟期花色素苷含量有所下降(发育中期积累型),如‘哈露红’‘大果黑桃’和‘乌黑鸡肉桃’。在与花色素苷合成相关的结构基因中,Pp CHS和Pp UFGT的表达量与成熟期积累型种质的花色素苷含量的变化趋势一致;而发育中期积累型种质,果肉中花色素苷的大量积累与所有结构基因的表达趋势一致。在4个调控基因中,只有Pp MYB10.1的表达水平较高,且表达模式同红肉桃种质两类花色素苷积累模式相似。【结论】根据桃果肉着色规律和花色素苷积累模式,8份红肉桃可以分为成熟期积累型和发育中期积累型种质。Pp CHS和Pp UFGT是成熟期积累型种质的关键结构基因,而Pp MYB10.1在供试的所有红肉桃种质花色素苷合成中起关键作用。
[Abstract]:[objective] to analyze the color type and molecular mechanism of main red peach germplasm in China. [methods] eight red peach germplasm and one white peach germplasm were selected as test materials, and the samples were collected one month after anthesis, respectively. The anthocyanins from different germplasm pulp were extracted with 2% formic acid methanol and their absorptivity values at 510nm and 700nm were measured respectively, and real-time fluorescence quantitative PCR(q RT-PCR method was used. The expression levels of 13 key structural genes and regulatory genes related to anthocyanin synthesis were determined. [results] according to the changing trend of anthocyanin content in the middle and late stages of fruit development, 8 red peach germplasms could be classified into two categories. The peak of anthocyanin synthesis in one type of germplasm appeared in the fruit mature stage, such as' Zhengyin 82-9 'Dahong Pao' black cloth bag 'red peach' and 'Tianjin water and honey'. The peak of anthocyanin synthesis in the other type of germplasm appeared in the middle stage of fruit development, and decreased in the mature stage (the accumulation of anthocyanin in the middle stage of development, such as' Harlow 'big fruit spade' and 'black chicken peach'). The expression of P p CHS and P p UFGT in the structural genes associated with anthocyanin biosynthesis was consistent with the change trend of anthocyanin content in the mature type germplasm, while in the middle developmental stage, the content of anthocyanin was similar to that of the middle stage accumulation type germplasm. The accumulation of anthocyanin in pulp was consistent with the expression trend of all structural genes. Among the four regulatory genes, only the expression level of P p MYB10.1 was higher. The expression pattern of anthocyanin was similar to that of red peach germplasm. [conclusion] according to the color pattern of peach pulp and anthocyanin accumulation model, 8 red peach cultivars can be divided into two types: mature accumulation type and middle stage accumulation type. Cytoplasm. P p CHS and P p UFGT are the key structural genes of the accumulative germplasm at maturity. P p MYB10.1 plays a key role in anthocyanin synthesis of all red peach germplasm tested.
【作者单位】：中国农业科学院郑州果树研究所;
【基金】：国家“863”计划(2013AA102606) 中国农业科学院科技创新工程[CAAS-ASTIP-2015-ZFRI-(01)]
【分类号】：S662.1

【参考文献】