儿童幽门螺杆菌毒力基因与抗生素耐药相关性研究

发布时间：2018-05-14 03:18

  本文选题：幽门螺杆菌 + 毒力基因　； 参考：《临床儿科杂志》2017年01期

【摘要】：目的探讨儿童幽门螺杆菌(H.pylori)临床分离株毒力基因与抗生素耐药之间的关系。方法从具有上消化道症状的90例患儿胃窦黏膜中分离培养H.pylori阳性菌株,采用E-test法和K-B法检测H.pylori对抗生素耐药性,聚合酶链反应(PCR)检测H.pylori cag A、vac A及ice A基因。结果在胃窦黏膜分离培养的90株H.pylori菌株中,8株(8.9%)对克拉霉素耐药,31株(34.4%)对甲硝唑耐药,12株(13.3%)对克拉霉素和甲硝唑二重耐药,39株(43.3%)对抗生素均不耐药,未发现对阿莫西林和呋喃唑酮耐药的H.pylori菌株;cag A基因阳性检出率为93.3%(84/90),vac As1a、vac As1c、vac Am1和vac Am2基因阳性检出率分别为77.8%(70/90)、22.2%(20/90)、32.2%(29/90)和67.8%(61/90),vac As1a/m1、vac As1a/m2、vac As1c/m1和vac As1c/m2基因阳性检出率分别为30.0%(27/90)、51.1%(46/90)、3.3%(3/90)和16.7%(15/90),ice A1和ice A2基因阳性检出率分别为87.7%(79/90)和7.8%(7/90)。H.pylori毒力基因型在克拉霉素耐药组、甲硝唑耐药组、克拉霉素+甲硝唑二重耐药组和对抗生素敏感组四组间的阳性检出率比较,差异均无统计学意义(P0.05)。结论儿童H.pylori临床分离菌株毒力基因型与抗生素耐药无相关性。
[Abstract]:Objective to investigate the relationship between virulence genes and antibiotic resistance of H. pylori strains in children. Methods H.pylori positive strains were isolated from antral mucosa of 90 children with upper digestive tract symptoms. The antibiotic resistance of H.pylori to antibiotics was detected by E-test and K-B methods. H.pylori cag Agnvac A and ice A genes were detected by polymerase chain reaction (PCR). Results among 90 strains of H.pylori isolated from gastric antral mucosa, 8 strains were isolated and cultured.) 31 strains were resistant to clarithromycin, 31 strains were resistant to clarithromycin, 12 strains were resistant to metronidazole, 12 strains were resistant to clarithromycin and metronidazole, 39 strains were resistant to clarithromycin and metronidazole, and 39 strains were not resistant to antibiotics. 鏈�鍙戠幇瀵归樋鑾�瑗挎灄鍜屽憢鍠冨攽閰�瑁�

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