LKB1基因表达与非小细胞肺癌预后的相关性研究

发布时间：2018-05-14 09:17

本文选题：非小细胞肺癌 + LKB1　；参考：《皖南医学院》2017年硕士论文

【摘要】：目的:探讨LKB1基因表达水平与NSCLC患者预后因素的相关性。方法:选取2015年2月至2016年8月弋矶山医院胸外科手术切除NSCLC癌组织石蜡包埋标本、新鲜组织标本23例为肺癌组,所有标本均经病理学检测,确诊为肺鳞癌、腺癌。同时选取同一患者癌旁正常肺部组织23例作为空白对照组。使用RT-PCR和Western-blot法检测癌组织及癌旁组织中LKB1基因mRNA和蛋白的表达量,并分析其与年龄、分化程度、临床分期、肿瘤大小、无进展生存期的关系。结果:在肺癌组,LKB1基因表达低于空白对照组,差异有统计学意义(P0.05),LKB1表达水平与患者年龄、临床分期、肿瘤大小无相关性(P0.05),与分化程度呈正相关(P0.05)。LKB1 mRNA高表达组肺癌患者中位无进展生存期为13.033月,低表达组中位生存期为5.267月,高表达组无进展生存期为(13.247±0.505)个月,低表达组无进展生存期为(6.426±1.251)个月,差异有统计学意义(P0.05)。LKB1蛋白高表达组肺癌患者中位生存期为12.833个月,低表达组中位生存期为8.467个月,LKB1蛋白高表达组无进展生存期为(13.487±1.079)个月,低表达组无进展生存期为(8.239±0.979)个月,差异有统计学意义(P0.05).结论:LKB1基因在非小细胞肺癌中的表达明显低于相应的癌旁组织,LKB1表达量与非小细胞肺癌分化程度相关,且LKB1低表达可能提示预后不良。
[Abstract]:Objective: to investigate the correlation between LKB1 gene expression and prognostic factors in NSCLC patients. Methods: paraffin embedded specimens of NSCLC cancer tissue were removed by thoracic surgery from February 2015 to August 2016. 23 cases of lung cancer were collected from fresh tissues. All the specimens were diagnosed as squamous cell carcinoma and adenocarcinoma by pathology. At the same time, 23 cases of normal lung tissue adjacent to cancer of the same patient were selected as blank control group. RT-PCR and Western-blot methods were used to detect the expression of LKB1 gene mRNA and protein in cancer tissues and adjacent tissues, and to analyze the relationship between LKB1 gene mRNA and protein expression and age, differentiation, clinical stage, tumor size and progression free survival. Results: the expression of LKB1 gene in lung cancer group was lower than that in blank control group. There was no correlation between tumor size and differentiation degree. The median progressive survival time of lung cancer patients with high expression of P0.05U. LKB1 mRNA was 13.03 3 months, the median survival time of low expression group was 5.267 months, and that of high expression group was 13.247 卤0.505 months. The progression free survival time of low expression group was 6.426 卤1.251 months, the median survival time of lung cancer patients with high expression of P0.05U. LKB1 protein was 12.833 months, the median survival time of low expression group was 8.467 months and that of high expression group of LKB1 protein was 13.487 卤1.079 months. The progression-free survival time of low expression group was 8.239 卤0.979 months, and the difference was statistically significant (P 0.05). Conclusion the expression of the LKB1 gene in non-small cell lung cancer is significantly lower than that in the adjacent tissues of non-small cell lung cancer. The low expression of LKB1 may indicate that the prognosis of NSCLC is poor.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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