斜带石斑鱼干扰素调节因子8基因的鉴定与表达模式分析

发布时间：2018-05-14 10:06

本文选题：干扰素调节因子 + 斜带石斑鱼　；参考：《厦门大学学报(自然科学版)》2017年02期

【摘要】：干扰素调节因子8(IRF8)是IRF家族转录因子中的一个成员.利用简并PCR和cDNA末端快速克隆(RACE)技术获得了斜带石斑鱼(Epinephelus coioides)IRF8基因(EcIRF8)全长cDNA序列,其编码422个氨基酸残基的蛋白质,含有脊椎动物IRF家族的特征性结构域,即N端保守的DNA结合结构域(DBD)和C端多样化的干扰素调控因子结合结构域(IAD),其中DBD中包含5个特征性的色氨酸残基和1个核定位信号.与绝大多数脊椎动物的IRF8基因相似,EcIRF8基因组序列也是9个外显子和8个内含子的结构.EcIRF8在斜带石斑鱼肝脏中高水平表达.活体聚肌胞苷酸刺激后6h,EcIRF8在头肾、中肾、胸腺、肝脏和肠中的表达量显著上调(p0.05),其中在中肾中上调幅度最大,是对照组的106倍;副溶血弧菌(Vibrio paraheamolyticus)刺激后24h,EcIRF8在肝脏中的表达量也显著上调(p0.05),但上调倍数仅为对照组的9.37倍.结果表明,EcIRF8基因可能主要参与斜带石斑鱼的抗病毒免疫反应.
[Abstract]:Interferon regulatory factor 8 (IF8) is a member of the IRF family of transcription factors. The full-length cDNA sequence of Epinephelus coioides)IRF8 gene EcIRF8 was obtained by degenerate PCR and rapid cloning of cDNA terminal RACE8. It encodes 422 amino acid residues and contains the characteristic domain of the IRF family of vertebrates. The N-terminal conserved DNA binding domain (DBD) and the C-terminal interferon regulatory factor binding domain (DBD) contain five characteristic tryptophan residues and one nuclear localization signal. Similar to the IRF8 gene of most vertebrates, the genomic sequence of EcIRF8 is also the structure of 9 exons and 8 introns. EcIRF8 is highly expressed in the liver of the oblique grouper. The expression of EcIRF8 in the head kidney, middle kidney, thymus, liver and intestine was significantly up-regulated at 6 h after stimulation of polycytidine in vivo, with the largest up-regulation of EcIRF8 in the middle kidney, 106 times of that in the control group. The expression of EcIRF8 in the liver of Vibrio parahaemolyticus was significantly up-regulated 24 h after stimulation, but the up-regulation was only 9.37 times of the control group. The results showed that the EcIRF8 gene might be involved in the antiviral immune response of the oblique grouper.
【作者单位】：集美大学水产学院;鳗鲡现代产业技术教育部工程研究中心(集美大学);福建省海洋生物资源开发利用协同创新中心;
【基金】：国家自然科学基金(U205123,31172438,31402329) 国家高技术研究发展计划(2012AA092201) 福建省自然科学基金(2012J06008,2014J05042) 厦门南方海洋研究中心科技项目(14PYY050SF03)
【分类号】：S917.4

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