福建省畲族CYP17A1基因及饥荒暴露与代谢综合征患者高血压的相关性研究

发布时间：2018-05-15 06:09

本文选题：CYP17A1基因 + 代谢综合征　；参考：《福建医科大学》2016年硕士论文

【摘要】：背景和目的:畲族是中国的少数民族之一,目前关于此民族高血压易感基因的研究尚缺乏,尤其在代谢综合征患者中。本研究的目的是探讨CYP17A1基因rs11191548与畲族代谢综合征患者的高血压的相关性,以及饥荒暴露和CYP17A1基因对高血压的交互作用。研究方法:本次研究纳入的研究对象均来自福建省宁德地区的畲族,根据ATPIII代谢综合征诊断标准,纳入代谢综合征患者698人,在此人群中根据高血压诊断标准分为高血压组和非高血压组,最终高血压组486人(男112人,女289人),非高血压组212人(女43人,男129人),彼此间无亲缘关系。采集一般体格检查和临床生化指标,同时收集外周血进行DNA的提取。采用Taqman探针法,通过Real-time PCR(ABI 7500)仪进行基因分型。在Windows SPSS 17.0(SPSS,Chicago,Illinois,USA)上进行数据处理。研究结果:(1)CYP17A1基因rs11191548位点CC基因型占5.2%,CT基因型占40.7%,TT基因型占54.1%,危险等位基因T在总体中出现的频率为74.4%,高血压组中TT基因型和T等位基因的频率均显著高于非高血压组(P0.05)。携带rs11191548 T风险等位基因者患高血压的风险是携带C等位基因的1.495倍(OR=1.495,95%CI:1.129,1.98,χ2=7.92,P=0.005)。(2)调整了年龄、性别、BMI后,CYP17A1基因rs11191548位点TT基因型组的SBP明显比CC基因型组高;(3)采用logistic回归模型分析CYP17A1基因位点rs11191548和高血压间的关系,在调整了年龄、性别、BMI、HDL-C、LDL-C、TG、TC、FBG、2h PG、吸烟史和饮酒史后,TT基因型携带者患高血压的风险明显增加,是CC基因型携带者的2.714倍(OR=2.714,95%CI:1.222,6.028),差异具有统计学意义(P=0.014)。在additive模型中,危险等位基因T携带者患高血压的风险是未携带者的1.6倍(OR=1.6,95%CI:1.174,2.81,P=0.003);在dominant模型中,TT基因型和CT基因型携带者患高血压的风险是CC基因型携带者的2.232倍(OR=2.232,95%CI:1.027,4.853,P=0.043)。在recessive模型中TT基因型携带者高血压的风险则是CC+CT基因型携带者的1.667倍(OR=1.667,95%CI:1.143,2.43,P=0.008)。(4)我们将1959年10月1日到1961年12月31日出生的人群定义为饥荒暴露组,1962年10月1日到1965年12月31日出生的人群定义为非暴露组,采用线性回归分析比较暴露因素和SBP、DBP的关系,在校正性别、BMI、HDL-C、LDL-C、TG、TC、FBG、2h PG、吸烟史和饮酒史后,暴露因素与SBP有关联(β=8.329,P=0.006),而与DBP无显著关系(β=1.731,P=0.295)。(5)采用线性回归分析,在校正性别、BMI、HDL-C、LDL-C、TG、TC、FBG、2h PG、吸烟史和饮酒史后,以非暴露+CC/CT组作为对照,暴露+TT组及暴露+CT/CC组与SBP有显著关联。但所有组别与DBP未发现明显相关。结论:综上所述,在畲族代谢综合征患者人群中,CYP17A1基因rs11191548 T/C与高血压有关,其等位基因T可能是高血压的风险等位基因。此外,胎儿期暴露于饥荒与收缩压显著关联,而且胎儿期暴露与rs11191548等位基因对收缩压存在交互作用。
[Abstract]:Background and objective: she is one of the minority nationalities in China. The purpose of this study was to investigate the relationship between CYP17A1 gene rs11191548 and hypertension in she nationality patients with metabolic syndrome, and the interaction between famine exposure and CYP17A1 gene on hypertension. Methods: the subjects of this study were all from she nationality in Ningde area of Fujian Province. According to the diagnostic criteria of ATPIII metabolic syndrome, 698 patients with metabolic syndrome were included. According to the diagnostic criteria of hypertension, 486 patients (112 males and 289 females) were divided into hypertension group and non-hypertension group according to the diagnostic criteria of hypertension. General physical examination and clinical biochemical indexes were collected and DNA was extracted from peripheral blood. Genotyping was carried out by Taqman probe and Real-time PCR(ABI 7500. Data processing was performed on Windows SPSS 17.0 Windows SPSS Chicago Illinois. Results the percentage of CC genotype at rs11191548 locus of CYP17A1 gene was 5.2and that of CT genotype was 40.7%. The frequency of risk allele T was 74.4%. The frequency of TT genotype and T allele in hypertension group was significantly higher than that in non-hypertension group (P 0.05). Patients with rs11191548 T risk allele were 1.495 times more likely to develop hypertension than those with C allele 1.495and 95% CI: 1.129% 1.98, 蠂 ~ 2 7.92% (P < 0.005) adjusted for age. The SBP of CYP17A1 rs11191548 locus TT genotype group was significantly higher than that of CC genotype group. Logistic regression model was used to analyze the relationship between CYP17A1 locus rs11191548 and hypertension. The risk of hypertension was significantly increased after smoking history and drinking history, and was 2.714 times higher than that of CC genotype carriers, and the difference was statistically significant (P 0.014). In additive model, the risk of hypertension in T carriers with risk allele was 1.6 times as high as that in non-carriers. The risk of hypertension in T carriers was 2.232 times higher than that in CC genotype carriers, and the risk of hypertension was 2.232 times higher in dominant model than that in CC genotype carriers, and the risk of hypertension was 2.23295CIw 1.0274.853P0.043. The risk of hypertension in T carriers was 1.174% and 2.81% respectively in dominant model, and the risk of hypertension in T carriers with CT genotype and TT genotype was 2.232 times higher than that in CC genotype carriers. In the recessive model, the risk of hypertension in TT genotype carriers is 1.667 times as high as that in CC CT genotype carriers, and 1.66795% CI: 1.143U 2.43 Pao 0.008K.) We define people born from October 1, 1959 to December 31, 1961 as famine exposed groups, and from October 1, 1962 to 1965, we define people born between October 1, 1959 and December 31, 1961 as famine exposure groups. The population born on December 31st is defined as the non-exposed group, A linear regression analysis was used to compare the relationship between exposure factors and DBP. After adjusted for 2 h PGG, smoking history and drinking history, exposure factors were correlated with SBP (尾 8.329P + 0.006), but not with DBP (尾 1.731P = 0.295). After 2 h PGG, smoking history and drinking history, there was a significant correlation between TT group and exposed CT/CC group, compared with non-exposed CC/CT group. However, no significant correlation was found between all groups and DBP. Conclusion: in conclusion, CYP17A1 gene rs11191548 T / C is associated with hypertension in the she nationality patients with metabolic syndrome, and its allele T may be a risk allele for hypertension. In addition, there was a significant correlation between fetal exposure to famine and systolic blood pressure, and there was interaction between fetal exposure and rs11191548 allele on systolic blood pressure.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R589;R544.1

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