苹果多肽激素及其编码基因MdCEP1调控拟南芥根系发育

发布时间：2018-05-15 09:07

本文选题：苹果 + 多肽激素　；参考：《园艺学报》2017年07期

【摘要】：植物多肽激素是一类经剪切后形成具有特殊功能的成熟的多肽。以‘嘎拉’苹果(Malus×domestica‘Royal Gala’)为研究试材,初步探讨多肽激素C-TERMINALLY ENCODED PEPTIDE1(CEP1)调控根系生长发育的机理,为苹果多肽激素的研究奠定基础。扩增获得多肽激素基因MdCEP1(MDP0000886459),其开放阅读框为366 bp,编码121个氨基酸。进化树分析表明,苹果MdCEP1与白梨的PbCEP1亲缘关系最近。表达分析显示MdCEP1在苹果根和茎中表达量最高,在叶和果实中相对较低;基因表达响应显示MdCEP1表达受到生长素的调控,低浓度生长素明显上调,而高浓度生长素显著抑制MdCEP1表达。外施人工合成小肽MdCEP1p~(Hyp)对拟南芥根系发育起到明显抑制作用,主根变短,侧根数减少。在拟南芥中异位表达MdCEP1,同样表现出主根变短,侧根数减少。qRT-PCR分析结果显示,外源MdCEP1p~(Hyp)处理和过表达MdCEP1均明显抑制了拟南芥根系生长素合成与转运相关基因的表达。研究结果表明MdCEP1在根系生长发育过程中起到了负调控作用。
[Abstract]:Plant polypeptide hormone is a kind of mature polypeptide with special function. Using Malus 脳 domestica'Royal Gala (Gala) as the experimental material, the mechanism of regulating root growth and development by polypeptide hormone C-TERMINALLY ENCODED PEPTIDE1 (CEP1) was preliminarily studied, which laid a foundation for the study of apple polypeptide hormone. The peptide hormone gene MDP0000886459 was amplified and its open reading frame was 366 BP encoding 121 amino acids. Phylogenetic tree analysis showed that MdCEP1 had the closest relationship with PbCEP1 of white pear. Expression analysis showed that MdCEP1 expression was the highest in apple roots and stems, but relatively low in leaves and fruits. The gene expression response showed that MdCEP1 expression was regulated by auxin, low concentration of auxin was up-regulated, and high concentration of auxin significantly inhibited the expression of MdCEP1. The growth of Arabidopsis thaliana roots was inhibited by the application of synthetic small peptide MdCEP1pHypyp. the main root became shorter and the number of lateral roots decreased. Ectopic expression of MdCEP1 in Arabidopsis thaliana also showed that the main root became shorter and the number of lateral roots decreased. The results of qRT-PCR showed that exogenous MdCEP1pHypHyp1 treatment and overexpression of MdCEP1 significantly inhibited the expression of auxin synthesis and transportation-related genes in Arabidopsis thaliana roots. The results showed that MdCEP1 played a negative role in root growth and development.
【作者单位】：山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部黄淮地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金项目(31601742) 教育部创新团队项目(IRT15R42) 山东省产业体系项目(SDAIT-06-03)
【分类号】：S661.1

【参考文献】