糯玉米opaque2基因近等基因系的创制

发布时间：2018-05-16 15:04

本文选题：糯玉米 + opaque基因　；参考：《作物学报》2017年12期

【摘要】：opaque2基因能够提高糯玉米赖氨酸、色氨酸等必需氨基酸的含量。用2种优质蛋白玉米(QPM)CA339和鲁2548自交系作为opaque2基因供体,25个品质较好的糯玉米自交系作为受体,利用回交技术和SSR分子标记辅助选择育种,创制糯玉米opaque2近等基因系。分子标记结果表明,不同受体以及两种供体间都存在多态性。5套创制成功的opaque2近等基因系的赖氨酸含量比其轮回亲本分别提高了59.0%、52.7%、48.5%、46.3%和61.9%,分别由0.308%、0.313%、0.309%、0.341%、0.323%提高到0.489%、0.478%、0.458%、0.498%、0.522%。本研究表明,可以利用此方法通过向不同遗传背景的多种受体导入opaque2,选取赖氨酸含量提高较大、透明表型的近等基因系,提高糯玉米的营养价值和经济价值。
[Abstract]:Opaque2 gene can increase the content of lysine, tryptophan and other essential amino acids in waxy corn. Two kinds of high quality protein maize inbred lines, QPMGGCA339 and Lu 2548, were used as opaque2 gene donors and 25 better quality waxy maize inbred lines as receptors. Backcross technique and SSR molecular marker assisted selection breeding were used to create opaque2 near-isogenic lines of waxy maize. The results of molecular markers showed that there were polymorphic 5sets of lysine content in the near isogenic lines of opaque2 in different receptors and between the two kinds of donors. The lysine content in the near isogenic lines of opaque2 was increased by 59.070% and 61.9%, respectively, from 0.3080.3090.3090.323% to 0.4890.4780.4580.49880.4980.4580.4982222, respectively. The content of lysine was increased from 0.3130.3090.323% to 0.4890.4780.4580.49880.4982222. The content of lysine was increased from 0.3080.3090.3090.323% to 0.4890.4780.45880.49822. The results showed that this method could be used to transfer opaque2 to multiple receptors from different genetic backgrounds and select near-isogenic lines with higher lysine content and transparent phenotype to improve the nutritional and economic value of waxy maize.
【作者单位】：中国农业科学院作物科学研究所/农业部遗传育种重点实验室;北京工商大学/北京市植物资源研究开发重点实验室;
【基金】：国家自然科学基金项目(31401390) 国家现代农业产业技术体系建设专项(CAR02-01)资助~~
【分类号】：S513

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