DNA甲基化酶抑制剂5-Aza-CdR对多能基因及甲基化相关基因在徒手克隆胚胎中表达模式的影响

发布时间：2018-05-17 09:48

本文选题：徒手克隆 + -Aza-CdR　；参考：《中国畜牧兽医》2016年09期

【摘要】：本试验通过实时荧光定量PCR方法对多能基因Oct4和Nanog及DNA甲基化相关基因Dnmt1和Tets在徒手克隆(handmade cloning,HMC)胚胎中的表达模式进行初步研究,并探讨5-Aza-CdR处理重构胚对这些基因表达模式的影响。结果显示,Oct4、Nanog和Tet3的表达在2细胞时期达到顶峰,Dnmt1和Tet2基因的表达随HMC胚胎发育而下降,而Tet1基因随HMC胚胎发育表达上升。使用5-Aza-CdR处理重构胚没有改变Oct4、Tet1和Tet3基因的表达模式,使Nanog基因在胚胎发育初期表达增加,Dnmt1和Tet2基因在胚胎发育初期表达降低。研究初步确立了Oct4、Nanog、Dnmt1和Tets基因在HMC胚胎的表达模式,5-Aza-CdR对重构胚的处理可对HMC胚胎的甲基化模式产生影响。
[Abstract]:In this experiment, the expression pattern of multipotent gene Oct4 and Nanog and DNA methylation related genes Dnmt1 and Tets in handmade cloning, HMC (handmade cloning, HMC) embryos were preliminarily studied by real time fluorescence quantitative PCR method, and the effect of 5-Aza-CdR treatment on the expression mode of these genes was discussed. At the peak of the 2 cell period, the expression of Dnmt1 and Tet2 genes decreased with the development of HMC embryos, while the Tet1 gene increased with the development of HMC embryos. The expression pattern of Oct4, Tet1 and Tet3 genes was not changed by 5-Aza-CdR treatment, and the Nanog gene increased in the early embryonic development, Dnmt1 and Tet2 genes were in the early embryonic development table. The study preliminarily established the expression patterns of Oct4, Nanog, Dnmt1 and Tets genes in HMC embryos, and the treatment of 5-Aza-CdR on the reconstructed embryos could affect the methylation patterns of HMC embryos.
【作者单位】：广西大学亚热带生物资源保护利用国家重点实验室;中国农业科学院广西水牛研究所广西水牛遗传繁育重点实验室;广西壮族自治区畜牧研究所;
【基金】：国家自然基金(31260552) 国家高技术研究发展计划(863)项目(2011AA100607)
【分类号】：Q78

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