转基因马铃薯检测用质粒标准分子研制

发布时间：2018-05-17 13:14

本文选题：转基因马铃薯 + 标准质粒　；参考：《中国农业科技导报》2017年02期

【摘要】：依据全球转基因作物商业化的强势发展态势,转基因马铃薯的普遍商业化已呈大势所趋。马铃薯是世界第四大食用作物,仅次于水稻、小麦和玉米。目前,全球获批商业化种植的转基因马铃薯45种,主要涉及抗虫、抗病毒及品质改良等性状。转基因技术在给人们带来巨大经济效益的同时,也引起了人们的担忧,主要包括食品安全及环境安全问题等,由此转基因生物产品成分的检测及监管的重要性凸显。在转基因检测领域,标准质粒以容易获得、纯度高、成本低与稳定性好等优点逐渐被广泛应用。在调研了所有商业化的转基因马铃薯的重要信息的基础上,针对这些转基因马铃薯的主要外源基因类型,构建含有抗马铃薯甲虫cry3A基因、胭脂碱合成酶启动子P-nos基因、抗马铃薯Y病毒pvycp基因及内标准基因尿苷二磷酸葡萄糖焦磷酸化酶PATA基因的多靶标质粒pB JGMM003。该质粒可用于大部分已批准转化体及部分在研转基因马铃薯的鉴定、检测与监管工作,解决了转基因马铃薯的阳性标准品匮乏等问题,保障了转基因马铃薯相关工作的顺利进行。
[Abstract]:According to the strong trend of commercialization of transgenic crops worldwide, the general commercialization of transgenic potato has been the trend of the times. Potatoes are the fourth largest edible crop in the world, after rice, wheat and corn. At present, 45 transgenic potato species have been approved for commercial cultivation in the world, mainly related to insect resistance, virus resistance and quality improvement. GMO technology not only brings great economic benefits to people, but also causes people's concern, including food safety and environmental safety, so the importance of detection and supervision of GMO products is highlighted. In the field of transgenic detection, standard plasmids have been widely used with the advantages of easy access, high purity, low cost and good stability. Based on the investigation of the important information of all commercial transgenic potatoes, the cry3A gene and the P-nos gene of the cochineal synthase promoter were constructed according to the main foreign gene types of the transgenic potato. Multitarget plasmid pB JGMM003 for resistance to potato Y virus pvycp gene and uridine diphosphate glucose pyrophosphorylase (PATA) gene. The plasmid can be used for the identification, detection and supervision of most of the approved transformants and some of the transgenic potatoes under study, thus solving the problem of shortage of positive standard materials for transgenic potatoes. It ensures the smooth progress of the related work of transgenic potato.
【作者单位】：辽宁大学生命科学院;中国农业科学院生物技术研究所;
【基金】：国家转基因生物新品种培育重大专项(2014ZX0801201B)资助
【分类号】：S532

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