烟草转录因子MYB12基因克隆及表达模式分析

发布时间：2018-05-17 21:39

本文选题：烟草 + 转录因子　；参考：《烟草科技》2017年08期

【摘要】：R2R3-MYB转录因子是植物中最大的转录因子家族之一,在植物生长发育和代谢活动中发挥着重要的调节作用。为明确MYB12转录因子在烟草中的生物学功能和调控机制,同源克隆了普通烟草MYB12基因(NtMYB12a和NtMYB12b),利用实时荧光定量PCR表征基因表达模式,结合生物信息学分析对基因功能和调控机制进行了初步预测。结果表明,NtMYB12a和NtMYB12b核苷酸序列一致性为92.91%,氨基酸序列一致性为87.91%。烟草MYB12基因结构高度保守,均由4个外显子和3个内含子组成。进化分析表明,普通烟草中NtMYB12a系由绒毛状烟草MYB12基因进化而来,而NtMYB12b则来源于林烟草。NtMYB12基因表达具有时空特异性,烟草在打顶以及盐、干旱、黑暗和磷饥饿等胁迫处理后,NtMYB12a和NtMYB12b基因表达差异明显,NtMYB12a基因表达水平在打顶处理下呈现上调趋势,在盐、黑暗和磷饥饿胁迫处理下显著下调,而在干旱胁迫下无明显变化;各种胁迫处理均可诱导NtMYB12b基因表达水平的提高,预示着NtMYB12基因在烟草响应各种非生物胁迫中发挥着重要的调控作用。
[Abstract]:R2R3-MYB transcription factor is one of the largest family of transcription factors in plants, which plays an important role in plant growth, development and metabolism. In order to clarify the biological function and regulatory mechanism of MYB12 transcription factors in tobacco, the MYB12 genes of common tobacco, NtMYB12a and NtMYB12bA, were cloned homologous, and their expression patterns were characterized by real-time fluorescence quantitative PCR. The gene function and regulation mechanism were preliminarily predicted by bioinformatics analysis. The results showed that the nucleotide sequence identity of NtMYB12a and NtMYB12b was 92.91, and the amino acid sequence of NtMYB12a was 87.91. The structure of tobacco MYB12 gene was highly conserved and consisted of 4 exons and 3 introns. Evolutionary analysis showed that NtMYB12a line evolved from the MYB12 gene of fluffy tobacco, while NtMYB12b originated from the spatio-temporal specificity of the expression of the. NtMYB12 gene in forest tobacco, and the tobacco was topping, salt and drought. The difference of NtMYB12a and NtMYB12b gene expression between dark and phosphorus starvation treatments was significant. NtMYB12a gene expression level increased under topping treatment, decreased significantly under salt, dark and phosphorus starvation stress, but had no obvious change under drought stress. All kinds of stress can induce the increase of NtMYB12b gene expression, which indicates that NtMYB12 gene plays an important role in tobacco response to abiotic stress.
【作者单位】：中国烟草总公司郑州烟草研究院;郑州大学;
【基金】：中国烟草总公司烟草基因组计划重大专项项目“烟草转录因子基因芯片研制及重要转录因子表达调控研究”[110201401011(JY-11)]
【分类号】：Q943.2;S572

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