草鱼GH基因3′部分序列多态性与生长性状及肌肉成分的相关性分析

发布时间：2018-05-17 22:08

本文选题：草鱼 + GH基因　；参考：《水产学报》2017年09期

【摘要】：为了解草鱼GH基因多态性与早期生长性状及肌肉成分的相关性,实验利用直接测序法从156尾草鱼GH基因的3′部分序列中共筛选到9个变异位点(分别命名为SNP1~SNP9:G2825A、G2914T、T2966G、A3002T、T3022C、A3301G、C3463T、C3547T、C3620T)。卡方检验结果显示,9个位点均未显著偏离Hardy-Weinberg平衡,且均表现为中等多态性(0.25PIC0.5);经连锁不平衡分析发现,SNP3、SNP4和SNP5位点为一组完美连锁不平衡,SNP2、SNP7、SNP9位点为一组完美连锁不平衡;GH基因3′部分序列5个位点单倍型分析共发现6种单倍型,其中Hap1(30.4%)所占的比例最高,Hap6(4.8%)所占比例最低。利用GLM及多重比较对草鱼GH基因中9个SNPs多态性与早期生长性状和肌肉成分进行相关性分析,发现SNP2和SNP3位点的纯合突变型在体质量、体长和粗脂肪性状上均显著高于野生型和杂合突变型;在双倍型分析时得出了相似的结果,同时含有SNP2和SNP3位点纯合突变的双倍型组合在生长和粗脂肪性状上均显著性高于其他组合。研究表明,草鱼GH基因中SNP2和SNP3位点与早期生长性状及肌肉成分存在显著性相关,可作为草鱼生长及肉质改良的候选辅助分子标记,并且为进一步对相关变异位点的功能验证奠定研究基础。
[Abstract]:In order to understand the relationship between GH gene polymorphism and early growth traits and muscle composition of grass carp, nine mutation sites were selected from 156 grass carp GH gene by direct sequencing (named SNP1: SNP9: G2825AG2914T, T2966GN, A3002T, T3022CA3301G, C3463T, C3547T, C3620T, respectively. The results were as follows: (1) in order to understand the relationship between GH gene polymorphism and early growth traits and muscle composition, nine mutation loci were selected from 156 grass carp GH genes (named SNP1: SNP9: G2825AG2914T1, respectively. The results of chi-square test showed that none of the 9 loci deviated significantly from the Hardy-Weinberg equilibrium. By linkage disequilibrium analysis, it was found that SNP3SNP4 and SNP5 loci were a group of perfect linkage disequilibrium (SNP2SNP7SNP9) loci, which were a group of perfect linkage disequilibrium genes. Six haplotypes were found by haplotype analysis of 5 loci in the 3 'partial sequence of GH gene. The proportion of Hap1 + 30.4) was the highest and the lowest was Hap64.8. Nine SNPs polymorphisms in GH gene of grass carp were analyzed by GLM and multiple comparisons. The homozygous mutants of SNP2 and SNP3 were found in vivo. The body length and crude fat were significantly higher than those of wild type and heterozygous mutant. The diploid combinations with homozygous mutations at both SNP2 and SNP3 loci were significantly higher than other combinations in growth and crude fat traits. The results showed that the SNP2 and SNP3 loci in the GH gene of grass carp were significantly correlated with the early growth traits and muscle composition, and could be used as candidate assistant molecular markers for growth and meat quality improvement of grass carp. It also lays a foundation for further research on functional verification of related mutation sites.
【作者单位】：上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室;
【基金】：现代农业产业技术体系专项(CARS-46-04) 上海市工程中心提升项目(16DZ2281200) 通威产学研项目(TW2014F003)~~
【分类号】：S917.4

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